New application of CTRP13 fat factor
A kind of adipokines, downstream technology, applied in the field of medicine
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Embodiment 1
[0071] After carotid balloon injury or sham operation injury in SD rats, CTRP13 (10 mg / kg·d) and vehicle (DMSO) were injected intraperitoneally, respectively. After 14 days, the mice were euthanized and a revascularization procedure was performed on the injured blood vessels. After fixed with 4% formaldehyde and embedded in paraffin, the blood vessels were sectioned. figure 1 Oil red-hematoxylin (HE) staining was used to detect the vascular morphology of rat carotid arteries after balloon injury in the sham operation + vehicle treatment group, balloon + vehicle treatment group, and balloon + CTRP13 treatment group. The result is as figure 1 shown. in figure 1 From left to right, the statistical charts of the average carotid artery thickness in the sham operation + vehicle treatment group, the balloon + vehicle treatment group, and the balloon + CTRP13 treatment group. Depend on figure 1 It can be seen that compared with the sham operation + vehicle treatment group, the ca...
Embodiment 2
[0073] After VSMC cells were treated with PDGF-BB (30ng / ml), they were stimulated with different concentrations of CTRP13 (100, 200, 300ng / ml) and carrier DMSO. figure 2 The EdU assay was used to detect the proliferation of VSMC cells for different concentrations of CTRP13. The first column is vehicle treatment group, the second column is PDGF-BB and vehicle treatment group, the third column is PDGF-BB and 100ng / ml CTRP13 treatment group, the fourth column is PDGF-BB and 200ng / ml CTRP13 treatment group, the fifth column is PDGF-BB and 300ng / ml CTRP13 treatment group. Depend on figure 2 It can be seen that after PDGF-BB treatment of VSMC cells, EdU-positive cells increased, and after further treatment with CTRP13, EdU-positive cells decreased, and with the increase of CTRP13 concentration, the amount of EdU-positive cells decreased. In conclusion, PDGF-BB treatment of VSMC cells can promote the proliferation of VSMC cells, while CTRP13 can inhibit the cell proliferation ind...
Embodiment 3
[0075] After VSMC cells were treated with PDGF-BB (30ng / ml), they were stimulated with different concentrations of CTRP13 (100, 200, 300ng / ml) and the carrier DMSO. After the cells were collected and the protein was extracted, the cyclins and tumor suppressors were detected by Western blot. protein expression, the results are as follows image 3 shown. Expression of cell cycle proteins (PCNA, CyclinD1) and tumor suppressor proteins (P27, P21) in VSMC cells under different treatments. Depend on image 3 It can be seen that after treatment with PDGF-BB, the expression of cyclins in VSMC cells increased and the expression of tumor suppressor proteins decreased; after stimulation with CTRP13, the expression of cyclins decreased and the expression of tumor suppressor proteins increased; while the expression of apoptosis-related proteins No significant changes.
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