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Application of verapamil in preparation of product for protecting cartilage cells and relieving osteoarthritis

A chondrocyte and osteoarthritis technology, applied in the field of medicine, can solve the problems of lack of specific drugs and unclear research, and achieve the effects of delaying the incidence of diseases, being convenient to use, and reducing the level of TXNIP.

Pending Publication Date: 2021-11-02
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the research on the internal mechanism of OA is still unclear, there is still a lack of specific drugs that can block the progression of OA at this stage

Method used

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  • Application of verapamil in preparation of product for protecting cartilage cells and relieving osteoarthritis
  • Application of verapamil in preparation of product for protecting cartilage cells and relieving osteoarthritis
  • Application of verapamil in preparation of product for protecting cartilage cells and relieving osteoarthritis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] 1. In vitro cell experiment of verapamil

[0102] 1.1 Method

[0103] Verapamil hydrochloride (B1867) was purchased from APExBIO; the ATDC5 chondrocyte line was provided by the Orthopedic Laboratory of the Ninth People's Hospital affiliated to Shanghai Jiao Tong University.

[0104] IC50 values ​​of Verapamil (TXNIP inhibitor) / DZNep (TXNIP agonist) were determined by CCK8. At the effective drug concentration, Verapamil (TXNIP inhibitor) / DZNep (TXNIP agonist) stimulated the ATDC5 chondrocyte cell line in vitro, and observed cell proliferation, differentiation and apoptosis. After drug stimulation, the level of TXNIP protein was detected by WB. Through ATDC5 in vitro high density, daily administration, stimulation for 7 days, Alician blue staining, and observation of extracellular matrix to verify the effect of Verapamil (TXNIP inhibitor) / DZNep (TXNIP agonist) on cartilage differentiation and maturation and cartilage extracellular matrix secretion influences. In vitro...

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Abstract

The invention relates to an application of verapamil in preparation of a product for protecting chondrocytes and relieving osteoarthritis. The product is a composition or a preparation containing verapamil, or an optical isomer thereof, or a pharmaceutically acceptable salt thereof. Compared with the prior art, the verapamil can promote the expression of an extracellular matrix of cartilage by efficiently reducing the level of TXNIP protein in the cartilage cells, so that the verapamil has the functions of protecting the cartilage cells and relieving osteoarthritis. Therefore, verapamil has an active ingredient which can safely and effectively protect cartilage cells and relieve osteoarthritis.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of verapamil for protecting chondrocytes and alleviating osteoarthritis. Background technique [0002] Osteoarthritis (OA) is an irreversible degenerative disease accompanied by progressive destruction of articular cartilage. Currently, about 7% of the world's population is affected by OA, and women are more severely affected. The latest research data shows that in the past 30 years, the number of people affected by OA has increased by 48% worldwide, and in 2019 statistics, OA is the 15th leading cause of disability in the world, accounting for 2% of total disability globally. This problem is likely to be exacerbated by an aging population and increasing obesity. Therefore, it is urgent to pay attention to and strengthen the research and exploration of OA. At present, symptomatic treatment such as health education, rehabilitation exercises, weight control, some non-steroidal a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/277A61P19/02A61P29/00A23L33/10
CPCA61K31/277A61P19/02A61P29/00A23L33/10A23V2002/00A23V2200/306
Inventor 赵杰杨二柱曹贤坤秦安
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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