Solid-phase extraction column selection method for measuring content of perchlorate by internal standard method

Abstract

The invention discloses a solid-phase extraction column selection method for determining the content of perchlorate by an internal standard method. The method comprises the following steps: (1) preparing and detecting a standard solution; (2) setting conditions of an ultra-high performance liquid chromatography-tandem mass spectrometry instrument; (3) calculating the absolute recovery rate; (4) comparing the absolute recovery rate values of perchlorate and 18O labeled perchlorate internal standard in the labeled sample extracting solution by different solid-phase extraction purification columns; (5) comparing response values and chromatograms of different solid-phase extraction columns to perchlorate and 18O labeled perchlorate internal standard in different labeled sample extracting solutions; (6) comparing clarity after the samples are purified by different purification columns; (7) detecting and verifying a sample adding standard recovery experiment by combining solid-phase extraction column purification with an internal standard method, and verifying the accuracy of the method; and (8) detecting and verifying an actual sample. The solid-phase extraction column suitable for measuring the content of the perchlorate through the internal standard method can be screened, the method is simple, and the analysis requirement for content detection of the perchlorate can be met.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a solid-phase extraction column selection method for determining perchlorate content by an internal standard method. Background technique [0002] Perchlorate is a highly diffusible and persistent pollutant. Human consumption of agricultural products containing perchlorate will cause thyroid dysfunction and endanger human health. Studies in recent years have shown that all kinds of food are generally contaminated by perchlorate, milk powder, grain, meat, vegetables, beverages, drinking water, tea, etc. have been detected with different concentrations of perchlorate. In recent years, analysis and detection techniques for perchlorate in various samples have been reported. [0003] Solid phase extraction is a commonly used purification method, which can remove impurities, avoid the interference of impurities on the target, improve the sensitivity of the a...

AI Technical Summary

Problems solved by technology

Commercially available solid-phase extraction columns are all expensive, so it is necessary to choose a method and screen to obtain a solid-phase extraction column that is suitable for the price and can meet the analysis and detection requirements.

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