Method for biosynthesizing phenolic acid compounds by using lignin and application of phenolic acid compounds

A technology for biosynthesis and phenolic acids, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of a large number of waste liquids, waste residues, environmental pollution, pollution, and high waste water treatment costs, and achieve less raw materials. , high efficiency and low cost

Active Publication Date: 2021-11-05
INST OF CHEM ENG GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, chemical methods are mostly used to prepare phenolic compounds from lignin, and lignin is synthesized by pyrolysis, gasification, hydrolysis, reduction and oxidation. Depolymerization into small molecule phenolic compounds, the preparation process uses a homogeneous metal salt catalyst and organic solvent, resulting in a large amount of waste liquid, waste residue, etc., resulting in subsequent separation and extraction operations, causing environmental pollution, and the cost of sewage and wastewater treatment in the later stage of production is high, while biosynthesis The method is environmentally friendly and economical

Method used

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  • Method for biosynthesizing phenolic acid compounds by using lignin and application of phenolic acid compounds
  • Method for biosynthesizing phenolic acid compounds by using lignin and application of phenolic acid compounds

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Embodiment 1

[0035] A method for utilizing lignin to biosynthesize phenolic compounds, comprising the following steps:

[0036] 1) Pick Bacillus siamensis (Bacillus siamensis) L5 single colony into LB liquid medium for pre-cultivation for 12h to obtain a premix, centrifuge the premix at 4000r / min for 2min, then discard the supernatant and leave the bacteria The body was washed with Tris-HCl solution and resuspended to obtain Bacillus siamese L5 bacterial solution, and the concentration of Bacillus siamese L5 in the bacterial solution was 10 5 ~10 6 CFU / mL, more preferably, the concentration of Bacillus siamese L5 in the bacterial liquid is 10 6 CFU / mL;

[0037] 2) Inoculate 500uL of Bacillus siamese bacterial liquid and 250uL of laccase into a 250mL conical flask of 50mL Tris-HCl solution, and add alkali lignin to obtain a mixed solution, so that the final concentration of alkali lignin in the mixed solution is 1000mg / L, the mixture was shaken and cultured for 1 day at a temperature of...

Embodiment 2

[0040]A method for utilizing lignin to biosynthesize phenolic compounds, comprising the following steps:

[0041] 1) Pick Bacillus siamensis (Bacillus siamensis) L5 single colony into LB liquid medium for pre-cultivation for 12h to obtain a premix, centrifuge the premix at 4000r / min for 2min, then discard the supernatant and leave the bacteria The body was washed with Tris-HCl solution and resuspended to obtain Bacillus siamese L5 bacterial solution, and the concentration of Bacillus siamese L5 in the bacterial solution was 10 5 ~10 6 CFU / mL;

[0042] 2) Inoculate 500uL of Bacillus siamese bacterial liquid and 500uL of laccase into a 250mL conical flask of 50mL Tris-HCl solution, and add alkali lignin to obtain a mixed solution, so that the final concentration of alkali lignin in the mixed solution is 1000mg / L, the mixture was shaken and cultured for 1 day at a temperature of 30 °C and a rate of 150 r / min, centrifuged to take the supernatant, added 50 mL of ethyl acetate an...

Embodiment 3

[0045] A method for utilizing lignin to biosynthesize phenolic compounds, comprising the following steps:

[0046] 1) Pick Bacillus siamensis (Bacillus siamensis) L5 single colony into LB liquid medium for pre-cultivation for 12h to obtain a premix, centrifuge the premix at 4000r / min for 2min, then discard the supernatant and leave the bacteria The body was washed with Tris-HCl solution and resuspended to obtain Bacillus siamese L5 bacterial solution, and the concentration of Bacillus siamese L5 in the bacterial solution was 10 5 ~10 6 CFU / mL;

[0047] 2) Inoculate 500uL of Bacillus siamese bacterial liquid and 1mL of laccase into a 250mL conical flask of 50mL of Tris-HCl solution, and add alkali lignin to obtain a mixed solution, so that the final concentration of alkali lignin in the mixed solution is 1000mg / L, the mixture was shaken and cultured for 1 day at a temperature of 30 °C and a rate of 150 r / min, centrifuged to take the supernatant, added 50 mL of ethyl acetate ...

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Abstract

The invention relates to the technical field of biosynthesis, and particularly discloses a method for biosynthesizing phenolic acid compounds by using lignin and application of the phenolic acid compounds. The method comprises the following steps: inoculating bacillus siamensis bacterial liquid and laccase into a Tris-HCl solution, adding alkali lignin into the mixture to obtain a mixed solution, carrying out shake culture on the mixed solution, performing centrifuging to obtain supernate, adding ethyl acetate and a hydrochloric acid solution into the mixture, and carrying out ultrasonic extraction, spin-drying and redissolving to obtain a phenolic acid compound, wherein the volume ratio of the bacterial liquid to the laccase is 1: (0.5-2) (v / v). The high-activity phenolic acid compound prepared by utilizing the method is high in content, the preparation method has the advantages of high efficiency, low cost and environmental friendliness, and the bacillus siamensis bacterial liquid and laccase are compounded according to the specific volume ratio, so that the prepared phenolic acid compound is high in content, and the phenolic acid compound also shows excellent antioxidant activity and antibacterial activity.

Description

technical field [0001] The invention relates to the technical field of biosynthesis, in particular to a method for biosynthesizing phenolic acid compounds by utilizing lignin and application thereof. Background technique [0002] Phenolic acid compounds, including p-coumaric acid, caffeic acid, ferulic acid, protocatechuic acid, gallic acid, ellagic acid, danshensu, salvianolic acid, rosmarinic acid, etc., which have excellent antioxidant, Anti-inflammatory, antibacterial, whitening and lightening, reducing blood lipids, anti-tumor, prevention and treatment of cardiovascular and cerebrovascular diseases and other biological activities, have a wide range of applications in medicine, food health care, cosmetics, animal and plant nutrition and other fields. [0003] Lignin is the most abundant aromatic compound in nature, and the earth can produce 6×10 per year 14 lignin is about t, which is a potential renewable carbon resource. In my country's industrial production, the ann...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/42C12P7/24C12P7/40C12P17/06A61K31/192A61K31/11A61P31/04A61P39/06C12R1/07
CPCC12P7/42C12P7/24C12P7/40C12P17/06A61K31/192A61K31/11A61P31/04A61P39/06
Inventor 肖盈陈佳志张靖雯麦裕良
Owner INST OF CHEM ENG GUANGDONG ACAD OF SCI
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