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Application of RNA binding protein Ythdf3, target spot for treating nerve axon injury and medicine

A technology that combines proteins and nerves, applied in the field of biomedicine, can solve problems such as inability to accurately obtain nerve damage repair

Pending Publication Date: 2021-11-09
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem in the prior art that it is impossible to accurately obtain the situation of nerve damage repair

Method used

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  • Application of RNA binding protein Ythdf3, target spot for treating nerve axon injury and medicine
  • Application of RNA binding protein Ythdf3, target spot for treating nerve axon injury and medicine
  • Application of RNA binding protein Ythdf3, target spot for treating nerve axon injury and medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Investigating the effect of interfering with Ythdf3 on the axon growth of DRG neurons, specifically including the following steps:

[0020] (1) Isolation and culture of DRG neurons:

[0021] Prepare the dissecting solution and place it in a Petri dish, add antibiotics and place on ice to pre-cool. Rats were anesthetized intraperitoneally, and the skin was cut from the tail along the spine to the head with surgical scissors, the entire spine was carefully removed, the lamina was opened from the neck, and all DRG tissues were pulled out with micro forceps and put into the dissection solution. After all the DRG tissues were taken out, the dissection fluid was discarded, and the tissues were rinsed twice with cell-grade PBS to remove excess tissues and blood. Discard the PBS, add 2ml collagenase (3mg / ml), and transfer the tissue and digestion solution to a 5ml centrifuge tube. The tissue was fully shredded with micro scissors, placed in a cell culture incuba...

Embodiment 2

[0029] Example 2: To investigate the effect of interfering with the expression of Ythdf3 on axon regeneration after sciatic nerve injury, the specific steps are as follows:

[0030] (1) Intrathecal injection of AAV virus

[0031] About 180 g male SD rats used in the experiment were provided by the Experimental Animal Center. Before the operation, intraperitoneal anesthesia is first performed, and then the hair around the back ilium is disinfected, then the skin is exposed with surgical scissors, the muscle tissue is cut along the ilium with ophthalmic scissors, the protruding spinous process is cut off with bone replacement scissors, and the glass electrode is inserted After the intervertebral space, Ythdf3 interference AAV virus (5X10^10 vg / body) was injected at a uniform speed with a micro-injector.

[0032] (2) Sciatic nerve pinch

[0033] The sciatic nerve crush was performed 14 days after the intrathecal injection of AAV virus. Firstly, intraperitoneal anesthesia was p...

Embodiment 3

[0039] Example 3: To investigate the effect of the overexpression of Ythdf3 on the regeneration of neuronal axons, the specific steps are as follows:

[0040] (1) Isolation and culture of DRG neurons:

[0041] Prepare the dissecting solution and place it in a Petri dish, add antibiotics and place on ice to pre-cool. Rats were anesthetized intraperitoneally, and the skin was cut from the tail along the spine to the head with surgical scissors, the entire spine was carefully removed, the lamina was opened from the neck, and all DRG tissues were pulled out with micro forceps and put into the dissection solution. After all the DRG tissues were taken out, the dissection fluid was discarded, and the tissues were rinsed twice with cell-grade PBS to remove excess tissues and blood. Discard the PBS, add 2ml collagenase (3mg / ml), and transfer the tissue and digestion solution to a 5ml centrifuge tube. The tissue was fully shredded with micro scissors, placed in a cell culture incubato...

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Abstract

The invention provides application of RNA binding protein Ythdf3, a target spot for treating nerve axon injury and a medicine, and relates to the technical field of biomedicine. The RNA binding protein Ythdf3 is used as a molecular target spot for regulating and controlling regeneration of nerve axon after peripheral and central nerve injury. According to the application of the RNA binding protein Ythdf3, research shows that expression of in-vitro and in-vivo interference Ythdf3 genes can remarkably inhibit growth of neuron axon. In addition, the regeneration process of sciatic nerve axon can be promoted by overexpression of Ythdf3 in DRG. Meanwhile, the inhibition effect of Ythdf3 gene interference on neuron axon can be inhibited by overexpression of a downstream important regulation and control target gene Foxo3 of Ythdf3. The research shows that Ythdf3 possibly influences growth of neuron axon by adjusting expression of the Foxo3 gene, and can become an important molecular target spot for nerve injury repair.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an application of RNA binding protein Ythdf3, a target for treating nerve axon damage and a drug. Background technique [0002] At present, the repair effect of nerve injury is not significant in patients with long-distance injury of peripheral nerve or central nervous injury. The regeneration ability of neuron axons is a key factor in nerve injury repair. Therefore, it is necessary to find a regulatory target that can effectively promote axon regeneration after nerve injury, and promote the therapeutic effect of nerve injury by regulating this target, so as to improve the current neurological The long-distance defect repair and incomplete functional recovery of peripheral nerves in injury treatment are conducive to the development of new treatment methods and strategies. Contents of the invention [0003] The purpose of the present invention is to solve the problem in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C12N15/864A61K45/00A61P25/00
CPCC07K14/47C12N15/86A61K45/00A61P25/00C12N2750/14143
Inventor 王东郑铁妹于彬
Owner NANTONG UNIVERSITY
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