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Novel Bjerkandera sp. SZ-4 and application thereof

A technology of SZ-4 and tobacco pipe bacteria, applied in the field of microorganisms, can solve the problem of single function, etc., and achieve the effect of low cost, powerful degradation function and simple separation steps

Active Publication Date: 2021-11-09
FUYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Corncob residues that have been extracted from xylo-oligosaccharides are rich in lignin, and corncob residues that have been stacked for a period of time contain a variety of microorganisms. Cellulose-degrading, or lignin-degrading, and none of the multifunctional microorganisms screened from corncob residue

Method used

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  • Novel Bjerkandera sp. SZ-4 and application thereof
  • Novel Bjerkandera sp. SZ-4 and application thereof
  • Novel Bjerkandera sp. SZ-4 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1.1 Experimental raw materials

[0040] The corncob residue extracted from xylo-oligosaccharides was purchased from Henan Yuanlong Biotechnology Co., Ltd.

[0041] 1.2 Separation method

[0042] After stacking the corncob residue naturally for 3 months, after sampling and mixing at multiple points, take 1g of the corncob residue into a sterilized Erlenmeyer flask, add 100mL of sterilized distilled water, and place it in a constant temperature shaking incubator at 170rpm for 12 hours.

[0043] Use a pipette gun to draw 200 μ L of corn cob residue sample extract, respectively in the bacterial primary screening medium (lignin 3g / L, agar 15g / L, KH 2 PO 4 1g / L, K 2 HPO 4 1g / L, MgSO 4 0.2g / L, FeSO 4 0.05g / L, (NH 4 ) SO 4 2g / L, MnSO 4 0.02g / L, CaCl 2 0.1g / L, natural pH value, sterilized at 121°C for 20 minutes) for coating culture, and the bacterial primary screening medium was placed in a 30°C constant temperature incubator for cultivation.

[0044] Pick fun...

Embodiment 2

[0051] Carry out the lignin degradation ability test to the pipe fungus SZ-4 that embodiment 1 screens out:

[0052] 1. Experimental culture medium

[0053] (1) Common media for bacterial strain purification

[0054] PDA medium: peeled potatoes 200g / L, sucrose 20g / L, agar 15g / L, natural pH, sterilized at 121°C for 20min;

[0055] PDA liquid medium: peeled potatoes 200g / L, sucrose 20g / L, natural pH, sterilized at 121°C for 20min.

[0056] (2) Screening medium for degrading ligninase

[0057] Lignin inorganic salt solid medium: lignin 3g, agar 15g, KH 2 PO 4 1g, K 2 HPO 4 1g, MgSO 4 0.2g, FeSO 4 0.05g, (NH 4 ) SO 4 2g, MnSO 4 0.02g, CaCl 2 0.1g, distilled water 1L, natural pH;

[0058] Lignin inorganic salt liquid medium: lignin 3g, KH 2 PO 4 1g, K 2 HPO 4 1g, MgSO 4 0.2g, FeSO 4 0.05g, (NH 4 ) SO 4 2g, MnSO 4 0.02g, CaCl 2 0.1g, distilled water 1L, natural pH;

[0059] Lignin inorganic salt-PDA liquid medium: peeled potato 200g, sucrose 20g,...

Embodiment 3

[0072] Carry out the ability test of degrading alizarin red to the pipe strain SZ-4 that embodiment 2 screens:

[0073] 1. Medium

[0074] (1) Common media for bacterial strain purification

[0075] PDA culture medium and PDA liquid culture medium are all the same as embodiment 2.

[0076] (2) Alizarin Red Degradation Effect Determination Medium

[0077] Alizarin red-PDA solid medium: peeled potato 200g, sucrose 20g, agar 20g, natural pH, alizarin red 0.100g, 1L distilled water;

[0078] Alizarin red-PDA liquid medium: 200g peeled potato, 20g sucrose, natural pH, 0.100g alizarin red, 1L distilled water;

[0079] PDA solid medium: 200g peeled potato, 20g sucrose, 20g agar, 1L distilled water.

[0080] 2. Determination of the ability of SZ-4 to degrade alizarin red

[0081] (1) Qualitative determination of plate

[0082] Fungal cakes were taken from the PDA solid medium and inoculated on the Alizarin Red-PDA solid medium respectively, and the fungi were cultured at 28°C fo...

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Abstract

The invention discloses a novel Bjerkandera sp. SZ-4 and application thereof, and belongs to the technical field of microorganisms. The invention discloses a strain of Bjerkandra sp. SZ-4, which has been deposited in Guangdong Microbiological Culture Collection Center on July 26, 2021 under a deposit number of GDMCC No: 61834 at Guangdong Microbiological Research Institute on Fifth Building, No. 59 Building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The Bjerkandra sp. SZ-4 disclosed by the invention is separated from corncob residues, not only has a strong lignin degradation capability, but also has a function of degrading various dyes, and can decolorize lignin and dyes, and change the current situation that most of the bacteria have a single function. The discovery of the strain has important significance on degradation of lignin and dyes in the environment.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a new strain of tobacco pipe fungus SZ-4 and its application. Background technique [0002] Corn is the most widely planted and the most productive cereal in the world, ranking first among the three cereal grain crops (wheat, rice, and corn). my country grows corn in four seasons, east, west, north, middle, spring, summer, autumn and winter. It is the unique "four seasons corn" hometown in the world. The annual output of corn is about 110 million to 160 million tons, and 20 million tons of leftover corn cobs can be produced. Therefore, there are a large number of corn cob resources that need to be developed and utilized urgently. [0003] With the rapid development of science and technology, the deep processing of corncobs has been continuously expanded, and a series of high value-added products such as furfural, xylose, xylitol, xylooligosaccharides, maltose, glucose, lactic acid binder...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/645A62D101/26A62D101/28A62D101/22
CPCC12N1/14A62D3/02A62D2101/26A62D2101/28A62D2101/22
Inventor 赵丰贝盛良全徐华杰吴福芳
Owner FUYANG NORMAL UNIVERSITY
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