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A bovine serum albumin-hydrophobically modified chitosan nanocapsule and preparation method thereof

A bovine serum albumin and chitosan nanotechnology, which is applied in the field of polysaccharides, can solve the problems of low drug loading rate and easy leakage, and can prevent the drug from being released too quickly, increase the biocompatibility, and improve the poor water solubility. Effect

Active Publication Date: 2022-07-19
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both chitosan and bovine serum albumin are composed of hydrophilic monomers. After being prepared into nano-carriers, the drug-loading rate of hydrophobic paclitaxel is not high, and leakage easily occurs in vivo

Method used

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  • A bovine serum albumin-hydrophobically modified chitosan nanocapsule and preparation method thereof
  • A bovine serum albumin-hydrophobically modified chitosan nanocapsule and preparation method thereof
  • A bovine serum albumin-hydrophobically modified chitosan nanocapsule and preparation method thereof

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Effect test

Embodiment 1

[0031] A preparation method of bovine serum albumin-hydrophobically modified chitosan nanocapsules, comprising the following steps:

[0032] (1) Preparation of hydrophobically modified chitosan: Weigh 2g of n-hexadecanoic acid and 8g of chitosan, add them to 1000g of dimethyl sulfoxide, stir for 10 minutes, control the temperature at 15°C, add 3g of 1-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 2.5 g of N-hydroxysuccinimide were stirred for 0.5 hours, heated to 60°C, stirred for 12 hours, and cooled to 20°C to obtain a mixed solution , the obtained mixed solution was poured into 1000g isopropanol, stirred for 20 minutes, filtered, the filter cake was washed with 50g ethanol-water mixture (the mass ratio of ethanol and water was 2:1), and the hydrophobically modified chitosan was obtained by vacuum drying ;

[0033] (2) Preparation of bovine serum albumin-hydrophobically modified chitosan nanocapsules loaded with antitumor drugs: take the hydrophobically mod...

Embodiment 2

[0035] A preparation method of bovine serum albumin-hydrophobically modified chitosan nanocapsules, comprising the following steps:

[0036] (1) Preparation of hydrophobically modified chitosan: Weigh 5g of n-hexadecanoic acid and 14g of chitosan, add to 2000g of ethanol, stir for 20 minutes, control the temperature at 25°C, add 5g of N,N-diisopropyl carbon Diimine and 4 g of N-hydroxysuccinimide were stirred for 1.5 hours, heated to 90° C., stirred for 24 hours, and cooled to 40° C. to obtain a mixed solution. The obtained mixed solution was poured into 3000 g of ethanol, stirred for 40 minutes, and filtered. , the filter cake was washed with 150g ethanol-water mixture (the mass ratio of ethanol and water was 4:1), and the hydrophobically modified chitosan was obtained by vacuum drying;

[0037] (2) Preparation of bovine serum albumin-hydrophobically modified chitosan nanocapsules loaded with antitumor drugs: take the hydrophobically modified chitosan obtained in step (1), ad...

Embodiment 3

[0039] A preparation method of bovine serum albumin-hydrophobically modified chitosan nanocapsules, comprising the following steps:

[0040](1) Preparation of hydrophobically modified chitosan: Weigh 4g of n-hexadecanoic acid and 10g of chitosan, add them to 1500g of dimethyl sulfoxide, stir for 15 minutes, control the temperature at 20°C, add 4g of 1-(3- Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 3.5 g of N-hydroxysuccinimide were stirred for 1 hour, heated to 80°C, stirred for 18 hours, and cooled to 30°C to obtain a mixed solution , the obtained mixed solution was poured into 1500g isopropanol, stirred for 30 minutes, filtered, the filter cake was washed with a mixture of 100g ethanol and water (the mass ratio of ethanol and water was 3:1), and the hydrophobically modified shell was obtained by vacuum drying Glycans;

[0041] (2) Preparation of bovine serum albumin-hydrophobically modified chitosan nanocapsules loaded with antitumor drugs: take the hydropho...

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Abstract

The invention relates to the technical field of polysaccharides, in particular to a bovine serum albumin-hydrophobically modified chitosan nanocapsule and a preparation method thereof, wherein chitosan is modified with n-hexadecanoic acid, and the anti-tumor drug-loaded chitosan is further prepared. BSA-Hydrophobically Modified Chitosan Nanocapsules. The nanocapsule prepared by the invention has a two-layer structure, the inner layer is hydrophobically modified chitosan, 9-O-n-octadecylberberine and paclitaxel, and the outer layer is bovine serum albumin. The nanomedicine can significantly enhance the inhibitory activity of paclitaxel on tumor cells.

Description

technical field [0001] The invention relates to the technical field of polysaccharides, in particular to a bovine serum albumin-hydrophobically modified chitosan nanocapsule and a preparation method thereof. Background technique [0002] Paclitaxel is one of the world-renowned antitumor drugs with a unique mechanism of action and broad antitumor activity. However, paclitaxel is slightly soluble in water. In order to improve its solubility, it needs to be used in combination with ethanol and polyoxyethylene castor oil, but the combination of ethanol and polyoxyethylene castor oil will increase the hypersensitivity reaction during chemotherapy. [0003] In the prior art, paclitaxel is usually made into liposome preparation, such as the preparation method of a kind of paclitaxel liposome preparation disclosed in Chinese patent CN101011357, using thin film dispersion method or spray drying method to prepare long-circulating paclitaxel liposome, with cholesterol. , distearoyl ph...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/69A61K47/62A61K31/337A61K47/61A61P35/00
CPCA61K47/6931A61K47/61A61K47/62A61K31/337A61P35/00
Inventor 秦大伟王利振
Owner QILU UNIV OF TECH
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