MiRNA marker for diagnosis and treatment of melanoma and related product thereof

A technology of melanoma and markers, applied in medical preparations containing active ingredients, drug combinations, antineoplastic drugs, etc., can solve problems such as lack of consensus, and achieve high diagnostic efficiency and good clinical application prospects

Pending Publication Date: 2021-11-12
北京百奥思科生物医学技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dysregulation of miRNA expression in cells can lead to the occurrence of various cancers including melanoma. The latest research shows that some miRNAs are abnormally expressed in samples of melanoma patients, but which miRNAs are related to the occurrence and development of melanoma and are There is still no consensus on drug targets that can be used to treat melanoma

Method used

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  • MiRNA marker for diagnosis and treatment of melanoma and related product thereof
  • MiRNA marker for diagnosis and treatment of melanoma and related product thereof
  • MiRNA marker for diagnosis and treatment of melanoma and related product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1 Screening for differentially expressed genes in melanoma

[0066] 1. Data source and processing

[0067]Public gene expression data and complete clinical annotations were searched in the Gene Expression Omnibus (GEO), and melanoma (Melanoma ) Gene expression dataset GSE20994, which includes blood sample data of healthy controls and melanoma patients, the sample size of the dataset is Case: Normal=35:22, and it is annotated with annotation files, multiple The average value of the probe corresponding to the same gene is taken as its expression level, and then the gene expression matrix file is obtained.

[0068] 2. Differential expression analysis

[0069] Use the "limma" package in R software to perform differential expression analysis on the above data, where the screening criteria for differentially expressed genes are: |log 2 FC|>0.5, adj.P value<0.05.

[0070] 3. Experimental results

[0071] The experimental results showed that a total of 397 differen...

Embodiment 2

[0074] Example 2 Verification of the diagnostic efficacy of miR-450a and miR-675

[0075] 1. Experimental method

[0076] Use the R package "pROC" to perform receiver operating characteristic (ROC) analysis, draw the ROC curve, and use the differentially expressed genes miR-450a, miR-675, and miR-450a+miR-675 screened in Example 1 as detection variables Analyze the AUC value, sensitivity and specificity of the above-mentioned indicators miR-450a, miR-675, miR-450a+miR-675 for the diagnosis of melanoma, when judging the diagnostic performance of a single miRNA, directly use the miRNA expression analysis. First call the pROC package, then read in the expression matrix constructed by the target miRNA, and run the command to draw the ROC curve, where the command uses a for loop and involves adding AUC, Thres (threshold), and Smooth (fitting curve) commands. When judging the diagnostic efficacy of miRNA combination, first use glmnet to perform Logistic regression analysis on miRN...

Embodiment 3

[0081] Example 3 Research on the relationship between the expression of miR-450a and miR-675 and the apoptosis of melanoma cells

[0082] 1. Experimental materials

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Abstract

The invention discloses a miRNA marker for diagnosis and treatment of melanoma and a related product thereof. The miRNA marker is a combination of miR-450a and miR-675. The product for diagnosis of the melanoma comprises a chip, a kit and a preparation, wherein the chip, the kit and the preparation comprise a probe for specifically recognizing the miR-450a and the miR-675 or a primer, probe or chip for specifically combining the miR-450a and the miR-675. According to the miRNA marker and the related product thereof, experimental verification shows that the miRNA marker has better diagnosis efficiency, and the combination of the miR-450a and the miR-675 has a synergistic effect, so that the miRNA marker and the related product thereof can be used for diagnosing and treating the melanoma.

Description

technical field [0001] The present invention belongs to the technical field of biomedicine. Specifically, the present invention relates to miRNA markers and related products used in the diagnosis and treatment of melanoma. More specifically, the miRNA markers described in the present invention include miR-450a, miR-675 . Background technique [0002] Melanoma, also known as malignant melanoma, is a malignant tumor formed by the mutation of melanocytes. Melanoma mostly occurs in the skin. It is the most aggressive and highly malignant skin tumor, and its mortality rate ranks first among skin malignancies. The incidence rate of malignant melanoma in the Caucasian population is 21.6 / 100,000; that of the African population is 1.0 / 100,000; the incidence rate of the Chinese population is slightly lower than that of the African population. However, the incidence rate in my country has doubled in recent years, with about 20,000 new cases each year. Liver metastases occur in 50%-80...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886A61K31/7088A61P35/00
CPCC12Q1/6886A61K31/7088A61P35/00C12Q2600/158C12Q2600/178C12Q2600/136
Inventor 崔明鹿杨志强孔云张仙
Owner 北京百奥思科生物医学技术有限公司
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