Method for industrially preparing high-purity ergothioneine

Abstract

The invention provides a method for industrially preparing high-purity ergothioneine. The method comprises the following steps of: carrying out hot water treatment on mycelium, carrying out solid-liquid separation, and performing collecting to obtain feed liquid containing ergothioneine; carrying out vacuum concentration, filtration, ultrafiltration, decoloration, desorption and concentration on the feed liquid containing ergothioneine; performing chromatographic purification on an ergothioneine desorption concentrated solution, collecting a target peak, performing concentrating until the solution is dry, and dissolving a product in water to obtain an ergothioneine sample solution; desalting and decolorizing the ergothioneine sample solution through ion exchange resin, and performing collecting to obtain a desalted ergothioneine concentrated solution; and carrying out coarse crystallization and recrystallization on the desalted ergothioneine concentrated solution, and performing drying to obtain high-purity ergothioneine crystal powder. According to the method, a large amount of high-purity ergothioneine is prepared from the mycelia of the pleurotus ostreatus CGMCC No.23071, the product quality of the ergothioneine is ensured. The method is particularly suitable for large-scale production of the ergothioneine, so that the requirements of the product in the fields of food, cosmetics, health care, medicine and the like are met.

Description

technical field [0001] The invention belongs to the related fields of biochemical products, food and medicine, and relates to a method for industrially preparing high-purity ergothioneine. Background technique [0002] Ergothioneine (L-Ergothioneine, EGT) is a rare natural chiral amino acid strong antioxidant, and the only natural 2-thioimidazole amino acid (2-thio-imidazole) found so far. It exists in the form of ketone, and the stability of thiol group is higher than that of mercapto group, so EGT will not spontaneously oxidize, and has good pH stability and thermal stability. Ergothioneine has the functions of scavenging free radicals, detoxifying, maintaining DNA growth, anti-radiation, anti-aging, etc. It is a unique cell physiological protection agent; secondly, the existence of the transporter ETT makes EGT the only antioxidant that can be transported between cells , can protect the nervous system, inhibit developmental defects, protect the liver and so on. In view ...

AI Technical Summary

Problems solved by technology

In the above purification method, acetonitrile solution is used as the mobile phase. Toxic acetonitrile is strictly restricted in the fields of cosmetics, food, and medicine. Secondly, the HILIC column directly purifies and extracts the ultrafiltration concentrate, and a large amount of pigments and impurities will cause contamination of the HILIC packing. , which seriously affects the service life of the HILIC column. The direct HILIC column purification of the leaching ultrafiltration concentrate reduces the single-batch processing capacity of the HILIC column, increases the number of comprehensive treatment batches, increases the use of organic solvents, and increases the cost sharply; finally, HILIC The dried sample after purification has different colors and unstable quality; in summary, the final sample obtained by this method limits the application of ergothioneine products in major fields