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Surface-immobilized bistable polynucleotide devices for the sensing and quantification of molecular events

A polynucleotide, bistable technology, applied in the direction of measuring device, scattering characteristic measurement, microorganism determination/inspection, etc.

Pending Publication Date: 2021-11-19
CALIFORNIA INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] However, existing high-sensitivity assays have technical details that make them difficult to multiplex multiple analytes
Furthermore, procedures for quantification using such assays often involve a series of dilution steps, which result in large sample

Method used

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  • Surface-immobilized bistable polynucleotide devices for the sensing and quantification of molecular events
  • Surface-immobilized bistable polynucleotide devices for the sensing and quantification of molecular events
  • Surface-immobilized bistable polynucleotide devices for the sensing and quantification of molecular events

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no. 1 example

[0060] Some embodiments of the invention show a first example of the measurement of one or more of the following characteristics: (1) Convert the analyte binding signal to a unique And can be enlarged DNA signals (the state of the device is changed to off). (2) The measurement of a variety of surface-based methods is used to sensitively measure the conversion of a large and flexible dual steady DNA apparatus when combined with a single analyte molecule. (3) Determination of the following capabilities: Multi-quantitative measurement of multiple analytes by PCR / sequencing or on the surface of the surface based on the double steady-state DNA nanostructure device . (4) Determination of digital quantification of protein molecules can be provided under the same scale as digital droplet PCR.

[0061]Some embodiments of the invention provide significant improvements to existing electrochemical assays. The folded assay (Lai, based on "Chapter Eight: Folding-and Dynamics-Based Electrochem...

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Abstract

Bistable devices are constructed using a polynucleotide platform for sensing molecular events such as binding or conformational changes of target molecules. Uses include measurement of target concentration, measuring the effect of environmental condition (such as heat, light, or pH) on the target, or screening a library for molecules that bind the target or modulate its biological function. Devices comprise three regions: a top lid, bottom lid, and flexible linker or hinge between them. A device has an open configuration in which the top and bottom lid are separated, and a closed configuration they are bound close together. Binding domains or variations of the target molecule are fixed to a device so that when the molecular event occurs, the device switches from open to closed, or vice versa, which generates a signal. Optimal device design is determined by the signal modality (optical or electronic) used to measure closure of surface-immobilized devices.

Description

[0001] Cross-reference related application [0002] This application is entitled to September 25, 2018 filed "bistable surface is fixed for sensing and quantitative molecular events polynucleotide device" of US Application Serial No. 16 / 350,115 a continuation application, in its entirety incorporated herein by reference. [0003] Statement Regarding Federally Sponsored Research or Development [0004] This invention was made with government support under grants from the National Science Foundation Grant No. CMMI1636364 and awarded by the Office of Naval Research funded the number N00014-14-1-0702. The Government has certain rights in this invention. Technical field [0005] The present invention relates to a structure for sensing and molecular events (e.g., binding events, conformational change, enzymatic modification or chemical modification) quantifying the target molecule may occur, for example where such quantification of molecular events may enable a single measure analyte c...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12P19/34
CPCC12Q1/6825G01N33/48721C12Q2525/197C12Q2537/125C12Q2563/179C12Q2521/301G01N21/1717G01N21/64G01N21/76G01N21/47G01N27/3277G01N2021/5903
Inventor A.戈皮纳思P.W.K.罗特蒙德
Owner CALIFORNIA INST OF TECH