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A strain of Pseudomonas balearica and its application in denitrification and phosphorus removal in river water

A Pseudomonas, nitrogen and phosphorus removal technology, applied in the field of environmental microorganisms, can solve the problems of inhibiting microbial activity, low C/P ratio, poor phosphorus accumulation effect, etc. Application potential, effectiveness in solving competitive problems

Active Publication Date: 2022-02-11
玉凰生态科技股份有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, highly polluted river black and odorous water has the characteristics of high phosphorus content and low C / P ratio, which is not conducive to biological phosphorus removal, and there is also the competition between phosphorus accumulating bacteria and denitrifying bacteria for carbon sources, resulting in the inability of phosphorus accumulating bacteria Effectively multiply into dominant bacteria, and the effect of phosphorus accumulation is very poor
In addition, the effect of biological nitrogen and phosphorus removal is easily limited by seasons, especially in northern regions, where the water temperature is maintained at 8-15 °C all year round, and it will be lower in winter, which seriously inhibits the microbial activity in the biological treatment system

Method used

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  • A strain of Pseudomonas balearica and its application in denitrification and phosphorus removal in river water
  • A strain of Pseudomonas balearica and its application in denitrification and phosphorus removal in river water
  • A strain of Pseudomonas balearica and its application in denitrification and phosphorus removal in river water

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Embodiment 1

[0024] Embodiment 1: Screening and performance determination of denitrifying phosphorus accumulating bacteria

[0025] In this embodiment, samples are taken from the bottom mud of the river channel, and they are inserted into the ADM-P medium for enrichment, separation and purification. Then, the strains obtained from the primary screening were inserted into the ADM-P medium for verification, and the high-efficiency denitrifying phosphorus-accumulating bacteria were obtained through secondary screening.

[0026] The denitrifying phosphorus accumulating bacteria of the present invention are obtained by separating and screening from a certain river bottom mud. The separation method was carried out as follows: Take 5 mL of bottom sludge into 45 mL of sterilized ADM-P enrichment medium. ADM-P medium formula: KNO 3 0.35 g / L, sodium succinate 1.2 g / L, MgSO 4 ·7H 2 O 0.5 g / L, CaCl 2 0.2 g / L, FeSO 4 ·7H 2 O 0.05 g / L, KH 2 PO 4 0.02 g / L, K 2 HPO 4 0.03 g / L. Then placed i...

Embodiment 2

[0029] Embodiment 2: Molecular biology identification of bacterial strain

[0030] Genomic DNA of Pb01 was extracted and used as a template to amplify 16S rDNA. Primers: 16S rDNA-8F: AGAGTTTGATCCTGGCTCA, see SEQ ID NO.2 in the gene sequence table; 1492R: GGTTACCTTGTTACGACTT, see SEQ ID NO.3 in the gene sequence table. PCR reaction system (20 μL): template DNA 5 μL, PCR Taqmix 10 μL, upstream and downstream primers 0.6 μL each, add ddH 2 O to 20 μL of reaction volume. PCR program: 94°C for 5 min, 94°C for 30 s, 50°C for 30 s, 72°C for 1 min, cycle 30 times, 72°C for 10 min, 16°C for 10 min. The purification and sequencing of PCR products were completed by Jieli Biomedical Technology Co., Ltd., and the sequencing sequences were registered in GenBank for homologous sequence comparison analysis. The phylogenetic tree of the strain was constructed using MEGA 7.0 software ( figure 1 ).

[0031] The effective nucleotide sequence length of Pb01 is 1024 bp, see the gene sequence li...

Embodiment 3

[0032] Example 3: Verification of nitrogen and phosphorus removal capabilities of bacterial strain Pb01

[0033] Pseudomonas balearica Pseudomonas balearica Pb01 has efficient phosphorus removal ability and can simultaneously remove nitrogen and phosphorus. In order to verify the above effect, the following experiment was carried out: the bacterial solution was inoculated in the ADM-P medium with a 2% inoculation amount, and cultured at 5 °C and 50 rpm constant temperature shaking, and the NO 3 - - Concentrations of N and TP. see results image 3 , in ADM-P medium, NO 3 - -N and TP degradation rates were 98.9% and 99.7%, respectively.

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Abstract

The invention discloses a strain of Pseudomonas balearicus, which is named as Pseudomonas balearicus ( Pseudomonas balearica ) Pb01, which was deposited in the China Center for Type Culture Collection on July 14, 2021, with the preservation number CCTCC NO: M2021885, involving the field of environmental microbiology technology. The present invention also discloses the application of the above-mentioned Pseudomonas balearica in denitrification and dephosphorization of river channel water. Pseudomonas balearica ( Pseudomonas balearica ) Pb01 has a strong low-temperature denitrification and phosphorus removal ability, which can effectively solve the problem of competition between denitrifying bacteria and phosphorus accumulating bacteria for carbon sources, and achieve the purpose of simultaneous denitrification and phosphorus removal. In the culture medium, its effect on NO 3 ‑ The removal efficiencies of ‑N and TP reached 98.9% and 99.7%, respectively.

Description

technical field [0001] The invention belongs to the technical field of environmental microbes, and in particular relates to a strain of Pseudomonas balearica and its application in nitrogen and phosphorus removal in river course water. Background technique [0002] With the rapid development of our country's economy, the problem of water pollution in our country is becoming more and more serious. The substandard discharge of sewage causes the pollution indicators (COD, N, P, etc.) of the river water to exceed the standard, causing the river water to turn black and smelly, and the river water to become eutrophic, which seriously affects the ecological environment and human production and life safety. Among them, for the removal of pollution indicator phosphorus, the existing research mainly uses chemical flocculation method and microbial method. Compared with chemical method, microbial phosphorus removal has the advantages of safety, non-toxicity and low cost. However, highl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/38C02F3/34C02F101/16
Inventor 邱建贺翁雯周国彪梁锡伟
Owner 玉凰生态科技股份有限公司
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