A kind of bioreactor for high-density culture of Lactobacillus ragweed and using method thereof
A bioreactor and high-density cultivation technology, applied in the field of bioreaction equipment, can solve the problems of resource waste, low density, and affecting the cultivation effect of Lactobacillus rags, and achieve the effect of fully utilizing and increasing the cultivation density
Active Publication Date: 2022-08-02
贵州省现代农业发展研究所
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Problems solved by technology
[0003] However, in the prior art, the existing bioreactor for cultivating Lactobacillus rags is limited by its own structure, which makes it inconvenient for the existing bioreactor for cultivating Lactobacillus rags to actually carry out internal culture medium and replacement adjustment, thereby making the internal cultivation difficult. The PH of the liquid fluctuates greatly, and the distribution of nutrients is uneven, so that the actual culture of Lactobacillus rags in the existing bioreactor for the cultivation of Lactobacillus rags is low, so that the actual use effect of the equipment is not good. The bioreactor for bacillus culture cannot uniformly fuse the adjustment reagents during the adjustment process of the internal culture medium, and needs to mix the reagents by stirring, but the actual adjustment efficiency of this method is low, and it also affects the actual production of Lactobacillus rags. The cultivation effect makes the actual use effect of the equipment not good, and at the same time, the existing bioreactor for the cultivation of Lactobacillus rags cannot be cultivated at high density, resulting in a waste of unnecessary resources
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Embodiment 1
[0040] Embodiment 1, refer to Figure 1-13 : a bioreactor for high-density cultivation of Lactobacillus ragus, comprising: a support mechanism 1, the support mechanism 1 includes a base 101, an adjustment part and a positioning part, the establishment of the base 101 provides the installation basis for other functional parts of the equipment, and the adjustment part and positioning components are arranged on the base 101, the storage mechanism 2, the storage mechanism 2 includes the storage frame 201, the liquid collecting frame 205 and the power components, the establishment of the storage frame 201 facilitates the installation and setting of other functional components of the equipment, and at the same time It can effectively protect the internal functional components, and the establishment of the liquid collection frame 205 can effectively carry out the temporary storage of the culture liquid. The establishment of the block 202 enables the telescopic rod 105 to rotate effec...
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The invention discloses a bioreactor for high-density cultivation of Lactobacillus ragus and a method for using the same, comprising: a support mechanism, the support mechanism includes a base, an adjustment part and a positioning part, the adjustment part and the positioning part are all arranged on the base, and the storage The storage mechanism includes a storage frame, a liquid collecting frame and a power component, the storage frame is arranged on the positioning member, a rotating block is fixedly connected to the bottom center of the storage frame, and the liquid collecting frame is arranged inside the storage frame. In the present invention, an appropriate amount of neutral culture is injected into the mixing frame, the carrier frame and the liquid injection frame, and two micro water pumps are activated at the same time, and then an appropriate amount of neutral culture liquid is injected into the liquid injection frame through the liquid injection pipe, so that the liquid injection frame After the liquid level of the internal neutral medium reaches a certain height, it can be injected into the infusion tube through the liquid level tube, so that the neutral medium can be effectively injected into the corresponding buffer frame through the infusion tube, and then the neutral medium inside the buffer frame is slowly cultured through the infusion cotton wick. Liquid is injected into the inside of the carrier frame.
Description
technical field [0001] The invention belongs to the technical field of biological reaction equipment, in particular to a bioreactor for high-density culture of Lactobacillus rouge and a method for using the same. Background technique [0002] Bioreactor refers to a reaction system in which naturally occurring microorganisms or microorganisms with special degradation ability are used to inoculate liquid or solid phases. [0003] However, in the prior art, the existing bioreactor for Lactobacillus ragweed cultivation is limited by its own structure, which makes it inconvenient to actually carry out the internal culture solution and replacement adjustment in the existing bioreactor for Lactobacillus rapatum cultivation, and then make the internal culture solution more inconvenient. The pH of the liquid fluctuates greatly, and the distribution of nutrients is uneven, so that the actual culture of Lactobacillus ragweed in the existing bioreactor for culturing Lactobacillus ragwee...
Claims
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IPC IPC(8): C12M1/36C12M1/34C12M1/26C12M1/00
CPCC12M23/48C12M23/50C12M23/38C12M33/04C12M41/26C12M41/42
Inventor陈曦黄道梅孟繁博郑秀艳
Owner贵州省现代农业发展研究所