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Method for evaluating biological safety of water supply system by using sodB gene

A biosafety and water supply system technology, applied in the field of sodB gene evaluation of biosafety of water supply system, can solve cumbersome and time-consuming problems, and achieve the effect of simple operation, easy mastery, fast and accurate measurement

Pending Publication Date: 2021-11-30
SHANGHAI MUNICIPAL WATER RESOURCES DEV & UTILIZATION NAT ENG CENT
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Problems solved by technology

Traditional methods are relatively cumbersome, time-consuming, and have certain detection limits

Method used

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  • Method for evaluating biological safety of water supply system by using sodB gene
  • Method for evaluating biological safety of water supply system by using sodB gene

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Embodiment Construction

[0016] The implementation of the present invention is described below through specific examples and in conjunction with the accompanying drawings, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific examples, and various modifications and changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention.

[0017] figure 1 It is a flow chart of the steps of a method for evaluating the biological safety of a water supply system by using the sodB gene of the present invention. like figure 1 Shown, the present invention a kind of method with sodB gene evaluation water supply system biosafety,

[0018] Including the following steps:

[0019] Step S1, taking a water sample, and after filtering t...

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Abstract

The invention relates to the field of drinking water safety and biological safety, and discloses a method for evaluating the biological safety of a water supply system by using an sodB gene. The method comprises the following steps: step 1, taking a water sample, filtering the water sample by using 0.22-[mu]m micropores, and carrying out total DNA extraction and purification on a filter membrane to obtain a DNA sample; step 2, carrying out quantitative PCR amplification by taking the DNA sample as a template; step 3, using a plasmid with a known concentration and containing an sodB gene as a standard substance, diluting the plasmid in a 10-fold gradient manner to serve as standard templates: 10<7>-10<2> copies / L, and simultaneously performing quantitative PCR amplification reaction on the standard templates and the sample to be detected according to step 2; step 4, after reaction is finished, drawing a fluorescent quantitative PCR standard curve according to a Ct value and a concentration of each concentration gradient; and step 5, calculating the copy number of the sodB gene in the sample according to the Ct value of the sample to be detected and the standard curve.

Description

technical field [0001] The invention relates to the field of drinking water safety bio-safety, in particular to a method for evaluating the bio-safety of a water supply system by using sodB gene. Background technique [0002] Drinking unclean water is one of the top four causes of diseases in the world. Ensuring the microbiological safety of drinking water is both an important component of the public health sector and a primary goal of water workers. In recent years, it has been found that the drinking water that has been disinfected and qualified in urban water purification plants, under the condition of ensuring a certain amount of residual chlorine, the phenomenon of heterotrophic bacteria in the pipe network water is still increasing, which will threaten the biological safety and water quality of drinking water. . Superoxide dismutase (SOD), widely present in animals, plants and microorganisms, catalyzes the disproportionation reaction of superoxide anion free radicals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851
CPCC12Q1/6851C12Q2531/113C12Q2545/113C12Q2563/107C12Q2537/16
Inventor 陈蕾姜巍巍黄佳姜蕾
Owner SHANGHAI MUNICIPAL WATER RESOURCES DEV & UTILIZATION NAT ENG CENT
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