Prebiotic composition and its use
A composition and prebiotic technology, applied in the field of prebiotic composition and its application
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no. 1 approach
[0114] The first embodiment relates to an ingestible composition comprising sphingosine gum prebiotic effective amount.
[0115] Prebiotics sphingosine gum effective amount can comprise all values 10g and between about 1g to about, for example, about 1.1, about 1.2, about 1.3, about 1.4, about 1.5, about 1.6, about 1.7, about 1.8, about 1.9, about 2.0, about 2.1, about 2.2, about 2.3, about 2.4, about 2.5, about 2.6, about 2.7, about 2.8, about 2.9, about 3.0, about 3.1, about 3.2, about 3.3, about 3.4, about 3.5, about 3.6 , about 3.7, about 3.8, about 3.9, about 4.0, about 4.1, about 4.2, about 4.3, about 4.4, about 4.5, about 4.6, about 4.7, about 4.8, about 4.9, about 5.0, about 5.1, about 5.2, about 5.3, about 5.4, about 5.5, about 5.6, about 5.7, about 5.8, about 5.9, about 6.0, about 6.1, about 6.2, about 6.3, about 6.4, about 6.5, about 6.6, about 6.7, about 6.8, about 6.9, about 7.0, about 7.1, about 7.2, about 7.3, about 7.4, about 7.5, about 7.6, about 7.7, about 7.8,...
Embodiment I
[0183] I. Preparation of I.HA / LA SPS and SOS
[0184] Preparation of sphingol gum polysaccharide.
[0185] The chain length of natural sphingol gum can be shortened using high pressure homogeneration by including the following procedure: (i) Powder of the sheath alcohol glue (eg, a frozen gum, Ditegue and rat ration) 1 % W / V is hydrated in 1 L deionized water to obtain hydrated homide alcohol glue solution; (ii) mechanically digest hydrated hood amine solution (10 times) under approximately 12,000 psi in an APV 1000 homogeneous machine to obtain Substancy solution; (iii) adds a sufficient amount of isopropyl alcohol to homogenized solution to obtain housing-homide alcohol gum polysaccharide precipitates; v) Sworm amoxy gum polysaccharide powder collected by dry and polishing. This procedure is used for the selected hood amines (eg, highlicazole, hygroglis, highlicidi gel, highacolurine pulse glue) to prepare the following sphingol gum polysaccharide samples, The showered in t...
Embodiment II
[0214] II. Embodiment II. Effects of hood amines (eg, natural hood amine glue, SPS and SOS) on the activity of the colorectal microbial group.
[0215] Samples containing 8 mg / ml (0.8% w / v) of SPS or SOS were diluted in factor 2 to provide samples containing 4 mg / ml (0.4% w / v) SPS or SOS. As described by Fehlbaum (2018), after using a vitro fermentation screen ("I-Screen") fermentation 24 hours, the sample 1-12 of the concentration of 4 mg / ml is measured more than two. Effect of fifteen intestinal microbial groups. Specifically, standard fecal microbial groups from 56 healthy adults (based on exclusion criteria are healthy) are used, which are prepared from frozen reserve to prepare overnight. After this, it was diluted in a microtiter plate, and the sample was added to the microtiter plate and then incubated at 37 ° C for 24 hours. After incubation, the culture samples were collected and processed for further analysis. In the 96-well culture plate, some holes are used i...
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