Function-integrated multi-level skin wound repair stent and preparation method thereof

A skin wound, multi-level technology, applied in the technical field of medical materials, can solve the problems of undiscovered three-layer structure multifunctional integration research reports, etc., achieve excellent biocompatibility and degradability, good biophase Capacitance and degradability, the effect of promoting cell growth

Active Publication Date: 2021-12-17
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no research report on the research method of preparing gradient protein and the multifunctional integration of three-layer structure in this study

Method used

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  • Function-integrated multi-level skin wound repair stent and preparation method thereof
  • Function-integrated multi-level skin wound repair stent and preparation method thereof
  • Function-integrated multi-level skin wound repair stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] This embodiment relates to a method for preparing a multi-level skin wound repair scaffold with integrated functions, and the specific steps include:

[0040] (1) Preparation of the upper layer of the stent: Dissolve 0.24g of polycaprolactone in 2mL of hexafluoroisopropanol, and magnetically stir until completely dissolved to obtain polycaprolactone spinning solution; take 2mL of polycaprolactone spinning solution , add a point electrode in the center of the ring electrode as a receiving device, draw the polycaprolactone spinning solution into a 5mL syringe, connect a 20-gauge stainless steel needle and connect it to a 10KV high voltage, the flow rate is 1mL / h, and the duration is 10min. Centripetally oriented nanofiber scaffold; then take 2mL of the gelatin / epidermal growth factor mixed solution, draw the mixed solution into a 5mL syringe, connect a No. 20 stainless steel needle and connect with 18KV high pressure, control the micro-injection pump to adjust the mixed so...

Embodiment 2

[0052] This example is an experiment to study the effect of bioactive protein gradient signal on the migration of epidermal cells. First, according to the method in Example 1 step (1), respectively prepare centripetal gradient coated fiber sheets, uniformly coated fiber sheets and pure glass slides, pure glass slides are used as blank control groups, and are respectively placed in the 24-well plate. In the holes, a polydimethylsiloxane (PDMS) cylinder with a diameter of 6 mm was placed in the center of the fiber scaffold in each hole, and the epidermal cells were evenly planted on the polydimethylsiloxane cylinders that were not covered. The peripheral area of ​​the fibrous scaffold was seeded with cells at a concentration of 5 × 10 5 cells / mL. After 6 hours of cell adhesion, the polydimethylsiloxane cylinder was removed to allow the cells to start migrating.

[0053] This example studies the effect of the gradient coating of biologically active protein protein on the migrat...

Embodiment 3

[0055] This embodiment is the antibacterial performance experiment of berberine in the middle layer of the bracket. According to the preparation method of the middle layer of the bracket in step (2) of Example 1, the berberine / gelatin nanofiber brackets containing 1%, 5% and 10% berberine were prepared respectively. Gelatin nanofiber scaffolds served as a control group. The antibacterial properties were determined by disc agar diffusion method. First, approximate the 1×10 8 coli suspension per mL was inoculated on a nutrient agar plate with a diameter of 9 cm. The middle layers of berberine scaffolds with different ratios were cut into circles with a diameter of 1.5 cm. Nanofiber pads were soaked with phosphate buffered saline (PBS) and then covered in the center of the agar plate. The whole process is carried out under sterile conditions. Then the agar plate was incubated at 37°C for 24h for further observation.

[0056] The preparation method of described 1%, 5%, 10% be...

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Abstract

The invention belongs to the technical field of medical materials, and relates to a function-integrated multi-layer skin wound repair stent and a preparation method thereof. The main structure of the repair stent is a three-layer stent of layer-by-layer deposition, the upper-layer stent is a centripetal-oriented polycaprolactone nanofiber stent loaded with a centripetal-gradient gelatin / epidermal growth factor bioactive coating, the middle-layer stent is a polycaprolactone / berberine nanofiber stent, and the lower-layer stent is a reticular nanofiber stent coated with an acellular dermal matrix. A designed biological material with the gradient structure has good biocompatibility and degradability, especially has the potential of promoting cell ingrowth, has important application in wound repair and regeneration tissue engineering, and provides a new thought for functionalization of nanofibers.

Description

Technical field: [0001] The invention belongs to the technical field of medical materials, and specifically relates to a functionally integrated multi-level skin wound repair scaffold and a preparation method thereof. The upper layer of the repair scaffold has a centripetal orientation topology and a centripetal gradient protein coating, which accelerates the migration of epidermal cells The antibacterial Chinese medicine berberine contained in the middle layer of the scaffold can play an effective antibacterial effect. The mesh scaffold coated with acellular dermal matrix on the lower layer of the scaffold can regulate the secretion of fatty liver cells. regeneration. Background technique: [0002] Wound healing is mainly composed of four stages: hemostasis, inflammation, proliferation, and remodeling, but a single non-surgical treatment can usually only promote wound healing. Specifically, traditional dressing management is excellent at accelerating the "hemostasis" phase...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/18A61L27/22A61L27/36A61L27/54A61L27/58A61L27/60D01D5/00
CPCA61L27/60A61L27/227A61L27/54A61L27/18A61L27/222A61L27/58A61L27/362D01D5/003D01D5/0015D01D5/0084A61L2300/216A61L2300/252A61L2300/414A61L2400/12C08L67/04
Inventor 吴桐周子艺冷向峰刘娜王元非张小佩宁胥超
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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