Magnetic micro-particle chemiluminescence kit for determining content of interleukin-8 in human serum

A chemiluminescence reagent and interleukin technology, which is applied in the field of magnetic particle chemiluminescence reagent kit and its preparation, can solve the problems of low degree of automation, complex and cumbersome operation, etc., and achieve the effect of low pretreatment requirements, simple operation and good stability

Pending Publication Date: 2021-12-17
BEIJING LEADMAN BIOCHEM
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the detection of IL-8 adopts the double-antibody sandwich ELISA method. In this method, the biotinylated anti-human IL-8 antibody binds to IL-8, and horseradish peroxidase is used to label avidin. Biotin and avidin are specific In combination, horseradish peroxidase cat...

Method used

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  • Magnetic micro-particle chemiluminescence kit for determining content of interleukin-8 in human serum
  • Magnetic micro-particle chemiluminescence kit for determining content of interleukin-8 in human serum
  • Magnetic micro-particle chemiluminescence kit for determining content of interleukin-8 in human serum

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Experimental program
Comparison scheme
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Embodiment 1

[0063] 1. Prepare various buffers and diluents, as follows:

[0064] 1. Preparation of calibrator with protein-containing buffer

[0065] Take a dry and clean beaker, peel and weigh it, add 500g of purified water, then add 2.5g of MOPS, 2.4g of magnesium sulfate, 2.8g of disodium hydrogen phosphate dodecahydrate, 1.8g of sodium azide, 10.0 1 g of bovine serum albumin and 20 g of trehalose are fully stirred until they are completely dissolved, and the pH is adjusted to 6.7 with hydrochloric acid or sodium hydroxide, and the purified water is adjusted to 1 L, and the pH is re-measured, and the pH is required to be between 6.7±0.1.

[0066] 2. Preparation of R1 reagent dilution

[0067] Take a dry and clean beaker, peel and weigh it, add 500g of purified water, then add 12.1g of Tris, 0.5g of sodium azide, 6.0g of sodium chloride, 9.5g of bovine serum albumin, 0.8g of Methyl cellulose, stir well until it is completely dissolved, adjust the pH to 7.4 with hydrochloric acid or so...

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Abstract

The invention relates to a magnetic micro-particle chemiluminescence kit for determining the content of interleukin-8 in human serum. The magnetic micro-particle chemiluminescence kit comprises a reagent R1, a reagent R2, a magnetic separation reagent, a calibration product and a quality control product, wherein the reagent R1 is prepared from a biotinylated IL-8 monoclonal antibody A and a reagent R1 diluent, the reagent R2 is prepared from an alkaline phosphatase labeled IL-8 monoclonal antibody B and a reagent R2 diluent, the magnetic separation reagent is prepared from commercial streptavidin magnetic beads and a magnetic separation reagent diluent, the calibration product and the quality control product are prepared from diluents containing IL-8 antigens with different concentrations and proteins, and a chemiluminescent substrate solution required in an experiment process is an alkaline phosphatase catalytic luminescence substrate solution. According to the invention, a chemiluminescence detection technology and a biotin-streptavidin system are combined for the first time to realize the purpose of quantitative detection of the content of IL-8 in a human serum sample, and a more accurate, precise, convenient, rapid and simple method is provided for clinical detection of IL-8 in human serum.

Description

technical field [0001] The invention relates to the field of medical diagnostic reagents, in particular to a magnetic particle chemiluminescence reagent for detecting the content of interleukin-8 (IL-8) in human serum by combining the principle of chemiluminescence detection technology with biotin-streptavidin system Boxes and methods of making them. Background technique [0002] Interleukins are a type of cytokines that are produced by and act on various cells. At least 38 interleukins have been discovered so far, named respectively as IL-1~IL-38, with complex functions; they play an important role in a series of processes such as the maturation, activation, proliferation and immune regulation of immune cells, and they also participate in Various physiological and pathological reactions of the body. The proliferation, differentiation and function of immune system cells are regulated by a series of cytokines. According to the structural homology of cytokines, they can be ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/543G01N33/58G01N33/532G01N33/535G01N21/76
CPCG01N33/6869G01N33/577G01N33/54326G01N33/581G01N33/532G01N33/535G01N21/76G01N2333/5421
Inventor 桂颖王鹏李晓娟
Owner BEIJING LEADMAN BIOCHEM
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