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Indoor air pollutant degradation by genetically modified plants

A plant and transgenic technology, applied in ferns, plant products, genetic engineering, etc., can solve the problem of unexpressed detoxification genes

Pending Publication Date: 2021-12-31
UNIV OF WASHINGTON
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, no detoxification genes have been expressed in houseplants

Method used

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  • Indoor air pollutant degradation by genetically modified plants
  • Indoor air pollutant degradation by genetically modified plants
  • Indoor air pollutant degradation by genetically modified plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0063] Materials and methods

[0064] Preparation of pothos

[0065] Golden pothos ivy plants obtained from a retail gardening store were grown at 50 μE m -2 sec -1 Grow in the light on a 16 hr day / 8 hr dark cycle in a plant room at 25°C. Stem segments were cut off, surface sterilized with 15% sodium hypochlorite, and washed 3 times with sterile deionized water. Sterilized stem segments were grown on solid Murashige and Skoog's (MS) basal medium 25 in culture vessels. After being cultivated under light for 1-2 months, new leaves and new roots grow on the stems, and these sterile plants are used to infect engineered Agrobacterium for genetic modification.

[0066] Vector construction and gene transformation

[0067] In order to genetically modify pottage dill, a gene vector containing transgenes 2e1, egfp, and hpt was constructed, each gene flanked by suitable promoter and terminator sequences for pottage pottage. The hpt gene encodes hygromycin B phosphotransferase...

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Abstract

A genetically modified houseplant capable of reducing levels of volatile organic carcinogenic compounds, such as formaldehyde, benzene, and chloroform, in the indoor air in urban homes of developed countries is disclosed. The plant expresses a detoxifying transgene, mammalian cytochrome P450 2e, and has shown sufficient detoxifying activity against benzene and chloroform. Air purifying biofilters utilizing the plants and methods of their use are also disclosed.

Description

technical field [0001] The present invention relates to transgenic houseplants capable of removing volatile organic carcinogens (VOCs) from indoor air and biofilters comprising such transgenic plants. [0002] Cross References to Related Applications [0003] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 792,726, filed January 15, 2019, which is incorporated herein by reference in its entirety. [0004] Statement Regarding Sequence Listing [0005] The Sequence Listing related to this application is provided in text format in lieu of a paper copy and is hereby incorporated by reference into the specification. The name of the text file containing the sequence listing is 70586_Seq_Final_2019-12-16.txt. The text file is 6.92KB; created on December 16, 2019; and is being submitted via EFS-Web along with the instructions. [0006] Statement of Government Licensing Rights [0007] This invention was made with Government support under CBET-143...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H9/00C12N15/82C12N15/87
CPCC12N15/90C12N15/8242C12N15/8259Y02A50/20A01G9/246B01D53/70B01D53/72B01D53/85B01D2257/2064B01D2257/7027B01D2257/708B01D53/46C12N9/0006C12N9/0071C12N9/1205
Inventor 张龙S·E·斯特兰德
Owner UNIV OF WASHINGTON