Application of N-acetylneuraminic acid in preparation of accelerant for promoting Roseburia proliferation

A technology of acetylneuraminic acid and accelerators, which is applied in nervous system diseases, applications, dairy products, etc., can solve problems such as no related reports, achieve high application value, prevent and/or treat intestinal flora disorders, The effect of promoting proliferation

Active Publication Date: 2022-01-11
MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the regulation and promotion of N-acetylneuraminic acid on the intestinal probiotic Roseburia

Method used

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  • Application of N-acetylneuraminic acid in preparation of accelerant for promoting Roseburia proliferation
  • Application of N-acetylneuraminic acid in preparation of accelerant for promoting Roseburia proliferation
  • Application of N-acetylneuraminic acid in preparation of accelerant for promoting Roseburia proliferation

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Experimental program
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Embodiment 1

[0023] Since the intestinal flora derived from the human body cannot completely colonize the mice, human feces were taken as intestinal flora samples after treatment, and the intestinal environment in the human body was simulated in vitro, and N-acetylneuraminic acid was observed. The effect on intestinal flora after intervention treatment, the specific operation process is as follows:

[0024] 1. Fecal sample processing

[0025] (1) PBS buffer preparation

[0026] Taking 1000ml as an example, weigh the following medicines with an electronic analytical balance and pour them into a 1000ml beaker, including: KH 2 PO 4 0.24g, Na 2 HPO 4 12H 2 O 2.90g, NaCl 8.00g, KCl 0.20g.

[0027] Use a graduated cylinder to measure 800ml of ultrapure water and pour it into a beaker, stir until completely dissolved, then measure the pH of the above solution with a pH meter, and adjust the pH to 7.4±0.05 with 0.1M HCl or NaOH.

[0028] Drain the above-mentioned completely dissolved and p...

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Abstract

The invention discloses application of N-acetylneuraminic acid in preparation of an accelerant for promoting Roseburia proliferation. An intervention experiment of the N-acetylneuraminic acid shows that the N-acetylneuraminic acid has the effects of regulating intestinal flora, particularly promoting Roseburia proliferation and increasing Roseburia abundance, so that the N-acetylneuraminic acid can be used as the proliferation accelerant; large-scale fermentation culture of Roseburia is carried out in vitro to prepare a corresponding probiotic agent, and the probiotic agent can also be used as a prebiotic drug, a functional food or a health food to act in vivo, so that intestinal flora disorder is effectively prevented and / or treated; and the N-acetylneuraminic acid can be used for treating various diseases related to Roseburia, including Crohn, gout, cirrhosis, constipation, depression and type 2 diabetes mellitus, namely, the new application of the N-acetylneuraminic acid is found for the first time, and the N-acetylneuraminic acid has extremely high application value in medical health care and industrial production.

Description

technical field [0001] The invention belongs to the technical field of probiotic proliferation promoters, and specifically relates to the use of N-acetylneuraminic acid in the preparation of promoters for promoting the growth of Roseburia. Background technique [0002] N-acetylneuraminic acid is the most widely distributed sialic acid (N-acylneuraminic acid) in nature, and it is mainly composed of glycoproteins, glycolipids or bacterial capsule substances in animal cell membranes or secretions. Is a negatively charged ion that gives saliva a smooth feel. In glycoproteins or glycolipids, N-acetylneuraminic acid is located at the non-reducing end of the sugar side chain due to the 2-position ketone group forming a glycosidic bond. Its negative charge or unique chemical structure makes it have various physiological specificity. Studies have found that N-acetylneuraminic acid has multiple functions such as anti-bacteria, virus, anti-inflammation, and improving immunity. After...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7012A61P1/00A61P1/08A61P19/06A61P1/16A61P25/24A61P1/10A61P3/10A21D2/18A23L33/125A23C9/156
CPCA61K31/7012A61P1/00A61P1/08A61P19/06A61P1/16A61P25/24A61P1/10A61P3/10A21D2/181A23L33/125A23C9/156A23V2002/00A23V2200/32A23V2200/322A23V2200/30A23V2200/328A23V2250/28
Inventor 刘庆军沈鹤霄张帆李国龙熊云刘慧敏
Owner MEI YI TIAN BIOLOGICAL MEDICINE WUHAN CO LTD
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