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Method for detecting aster donoryzicola or aster marginata

A detection method and technology for aster, applied in the field of analysis of natural plant medicines, can solve the problems of unguaranteed safety and effectiveness of clinical medicines, confusion of the basis, and unfavorable control and evaluation of the overall quality of aster medicinal materials.

Pending Publication Date: 2022-01-11
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Aster is a recognized "difficult group" in taxonomy. As the main source of Tibetan Aster, it also faces the problem of basic confusion, which is not conducive to the overall quality control and evaluation of Tibetan Aster. to guarantee

Method used

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  • Method for detecting aster donoryzicola or aster marginata
  • Method for detecting aster donoryzicola or aster marginata
  • Method for detecting aster donoryzicola or aster marginata

Examples

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Embodiment 1

[0024] Example 1 Detection method of Aster edulis and Aster ciliate

[0025] Chromatographic conditions:

[0026] Chromatographic column: (2.1×100 mm, 1.8 µm) ACQUITY UPLC R HSS C18; mobile phase: acetonitrile (A)-0.1% aqueous formic acid (B); gradient elution, 0-5 min, 10%-12% A; 5~19 min, 12%~16% A; 19~26 min, 16%~24% A; 26~28 min, 24%~24% A; 28~30min, 24%~30%A; 35~45 min, 30%~45% A; volume flow rate 0.2 mL.min-1; column temperature 35°C; detection wavelength 325 nm

[0027] Extraction and Separation

[0028] Weigh 300g each of Aster dongeruo and Aster phyllosa, add 1000ml 70% ethanol to reflux to extract

[0029] Take 3 times, the time is 1h, and concentrate under reduced pressure to obtain the extract. Add distilled water to dissolve, then pass through HP-20

[0030] Macroporous resin separation, first eluted with 15% ethanol, discarded, then eluted with 70% ethanol, collected eluate,

[0031] Concentrate and dry to obtain 14.84g and 21.79g of the crude extracts of A...

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Abstract

The invention provides a method for detecting aster donoryzicola or aster marginata by using UPLC. According to the method, specific chromatographic conditions are adopted in the UPLC, more than 12 chromatographic peaks can be effectively separated and detected in the aster donoryzicola or the aster marginata, qualitative and quantitative detection can be carried out on the 12 chromatographic peaks, and an effective method is provided for quality control of the aster donoryzicola or the aster marginata.

Description

technical field [0001] The invention relates to an analysis method for natural plant medicine. Background technique [0002] Tibetan aster is mainly used as medicine with a variety of plants of the genus Asteraceae, which has rich sources and is a typical multi-based original Tibetan medicinal material. Li Biao et al. found that there are more than 70 species of medicinal plants in 28 genera of Asteraceae distributed in the Qinghai-Tibet and Gannan regions alone, with With a high research potential, Li Weiping concluded that it is necessary to carry out research on the Aster species on the Qinghai-Tibet Plateau after conducting biological research on Asteraceae plants. Aster is a recognized "difficult group" in taxonomy. As the main source of Tibetan Aster, it also faces the problem of basic confusion, which is not conducive to the overall quality control and evaluation of Tibetan Aster. to guarantee. Contents of the invention [0003] Based on the current confusion in t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/86
Inventor 张志锋苏学燕
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES