Oxytocic for serissa serissoides and artificial breeding method

A technology of oxytocic agent and high body, which is applied in the direction of pharmaceutical formula, medical preparations containing active ingredients, animal husbandry, etc., can solve the problem of low egg laying rate, fertilization rate, hatching rate and fry survival rate, difficulty in artificial domestication, reproductive Problems such as dysfunction, to achieve the effect of improving quality and hatching rate, easy factory breeding, and accelerating spawning time

Inactive Publication Date: 2022-01-14
GUANGDONG ZHANJIANG PROVINCIAL LAB OF SOUTHERN MARINE SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the large-scale cultivation of tall amberjacks has not yet formed in China, mainly because the number of tall amberjacks caught in the sea is small, and it is difficult to adapt to the artificial breeding environment, and it is difficult to artificially domesticate them. When they are raised in ponds, they may show Severe reproductive dysfunction, difficulty in spontaneous spawning
In addition, the artificial breeding technology of tall amberjack is immature, and the spawning rate, fertilization rate, hatching rate and fry survival rate are low, which seriously restricts the factory farming of tall amberjack

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] This embodiment provides an oxytocic agent. Oxytocic agents include gonadotropin-releasing hormone agonist (GnRHa), dioconone (DOM) and 3-year-old grouper pituitary extract. The concentration of GnRHa is 1 mg / ml, the concentration of DOM is 50 mg / ml, and the concentration of the 3 age grouper pituitary gland extract is 1 mg / ml normal saline; the GnRHa, DOM and 3 age grouper The volume ratio of fish pituitary extract is 3:6:1.

[0074] Among them, the 3 age grouper pituitary gland extract is ground by first grinding the 3 age grouper pituitary gland, and then blending with normal saline, the ratio of pituitary gland and normal saline is 1mg pituitary gland / ml normal saline, centrifugal After taking the supernatant, the 3-year-old oblique grouper pituitary extract with a concentration of 1 mg pituitary / ml normal saline can be obtained, and the extract can be diluted twice with normal saline to obtain a concentration of 0.5 mg brain. Pituitary gland / ml physiological sali...

Embodiment 2

[0083] In order to explore the fertilization rate and hatching rate of fertilized eggs after induced labor in the process of artificial breeding of tall amberjack, the following experiments were carried out:

[0084] (1) During the 6 hours from 19:00 on May 11 to 01:00 on May 12, timely catch the fertilized eggs that floated up and put them in the hatching bucket, randomly sampled and counted, and counted the number of fertilized eggs; meanwhile, cleaned up the sunken eggs in time Eggs were randomly sampled and counted to count the total number of eggs. Fertilization rate = number of fertilized eggs / total number of eggs × 100%. The water temperature in the hatching bucket is kept at about 27±0.2°C, the salinity is kept at about 34.5±0.5‰, the pH is kept at about 8±0.1, and the dissolved oxygen is kept at about 7±1mg / L. Micro-flow water hatching or micro-flow water aeration hatching is required. , with a density of 15,000 grains / m 3 .

[0085] (2) The counted number of ferti...

Embodiment 3

[0087] In order to explore the hatching rate of fertilized eggs during the artificial breeding of tall amberjack, the following experiments were carried out:

[0088] (1) From 22:00 to 22:30 on May 12, randomly sample and count the hatched fry, count the number of hatched fry, and calculate the hatching rate. Hatching rate = number of hatched fry / number of fertilized eggs × 100%. The water temperature in the hatching bucket is kept at about 25±0.2°C, the salinity is kept at about 34.5±0.5‰, the pH is kept at about 8±0.1, and the dissolved oxygen is kept at about 7±1mg / L. Micro-flow water hatching or micro-flow water aeration hatching is required. , with a density of 15,000 grains / m 3 .

[0089] (2) The number of hatched fry counted is about 417,000, and the hatching rate is 43.9%.

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Abstract

The invention discloses an oxytocic for serissa serissoides and an artificial breeding method. The oxytocic comprises a gonadotropin-releasing hormone agonist (GnRHa), DOM and 3-4-year-old epinephelus coioides pituitary extract, and can achieve a good oxytocic effect. The artificial breeding method comprises the steps of backup parent fish breeding, oxytocic parent fish selection, anesthesia and artificial oxytocic, fertilization and incubation, fry breeding and the like. Nutrients of parent fishes are strengthened through fresh baits such as squids, the oxytocic is injected after anesthesia, the spawning time of serissa serissoides is shortened, the quality of fertilized eggs is improved, the hatching rate of the fertilized eggs is increased, the serissa serissoides are fed with proper baits in all growth stages, and the survival rate of the serissa serissoides is increased. By adopting the method for artificial breeding of serissa serissoides, the fertilization rate, the hatching rate and the survival rate are high, and industrial breeding of serissa serissoides is easy.

Description

technical field [0001] The invention belongs to the technical field of fish oxytocin methods, and in particular relates to an oxytocin agent and an artificial breeding method for tall amberjack. Background technique [0002] Seriola dumerili belongs to Perciformes, Perciformes, Trevalidae, and Amberis genus. It is a carnivorous fish in the middle and upper layers of warm water. It is mainly distributed in subtropical regions such as the Mediterranean Sea, the Eastern Atlantic Ocean, and the Southeast China Sea. region, with fast growth, good meat quality and high value, it is a worldwide deep-sea economic fish. Amberjack is a dioecious fish. The male and female groups develop synchronously. The age of first sexual maturity is 3 to 4 years old. It lays floating eggs and can lay eggs multiple times. The breeding season is between late spring and early autumn. [0003] At present, the large-scale cultivation of tall amberjacks has not yet formed in China, mainly because the nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/09A61P15/08A01K61/10A01K61/17A61K31/454A61K35/60
CPCA61K35/60A61K31/454A61K38/09A61K9/0019A61P15/08A01K61/10A01K61/17A61K2300/00Y02A40/81
Inventor 朱春华邓秋霞黄洋张博赵娜刘子俊何睿祺茹笑影朱旭枫胡琴
Owner GUANGDONG ZHANJIANG PROVINCIAL LAB OF SOUTHERN MARINE SCI & ENG
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