Unlock instant, AI-driven research and patent intelligence for your innovation.

Anti-myoglobin antibody, and kit and method for detecting myoglobin

A myoglobin and antibody technology, applied in chemical instruments and methods, anti-animal/human immunoglobulin, biological testing, etc.

Pending Publication Date: 2022-01-14
DONGGUAN PENGZHI BIOTECH CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there are some deficiencies in the sensitivity, specificity, and affinity of monoclonal antibodies used to detect myoglobin, and there is still a lot of room for improvement. There is still a strong demand for monoclonal antibodies to detect myoglobin in this field

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anti-myoglobin antibody, and kit and method for detecting myoglobin
  • Anti-myoglobin antibody, and kit and method for detecting myoglobin
  • Anti-myoglobin antibody, and kit and method for detecting myoglobin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] In this example, the restriction endonuclease and Prime Star DNA polymerase were purchased from Takara Company. MagExtractor-RNA extraction kit was purchased from TOYOBO Company. BD SMART TM RACE cDNA Amplification Kit was purchased from Takara Company. The pMD-18T vector was purchased from Takara Company. Plasmid extraction kit was purchased from Tiangen Company. Primer synthesis and gene sequencing were performed by Invitrogen.

[0112] 1 Construction of recombinant plasmids

[0113] (1) Antibody gene preparation

[0114] The mRNA was extracted from the hybridoma cell line (5G9) of the anti-myoglobin monoclonal antibody, and the DNA product was obtained by RT-PCR. The product was inserted into the pMD-18T vector after adding A with rTaq DNA polymerase, and transformed In the DH5α competent cells, after the growth of colonies, four heavy chain and light chain gene clones were respectively taken and sent to a gene sequencing company for sequencing.

[0115] (2) ...

Embodiment 2

[0135] Antibody performance testing

[0136] (1) Activity detection of the antibody and its mutants in Example 1

[0137] Further analysis, the heavy chain variable region of the myoglobin monoclonal antibody (WT) of Example 1 is shown in SEQ ID NO: 12, wherein, wherein, the amino acid sequence of each complementarity determining region is as follows:

[0138] CDR1-VH: G-Y-T-Y(X1)-T-E(X2)-F-S-V(X3)-H;

[0139] CDR2-VH: W-I(X1)-N-S(X2)-E-T-S-E(X3)-P-T-Y-G(X4)-D-D-F;

[0140] CDR3-VH: A-R-R-A(X1)-Y-Y-L(X2)-F-G;

[0141] Its light chain variable region is shown in SEQ ID NO: 11, wherein the amino acid sequence of each complementarity determining region of the light chain is as follows:

[0142] CDR1-VL: R(X1)-A-S-K(X2)-S-V-D-F(X3)-N(X4)-G-D-S-Y;

[0143] CDR2-VL: A-A-S-Q(X1)-R-E(X2)-S;

[0144] CDR3-VL: Q-H(X1)-S-N-E-E(X2)-P-Y.

[0145] On the basis of the myoglobin monoclonal antibody in Example 1, mutations were carried out at sites related to antibody activity in the com...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an anti-myoglobin antibody, and a kit and a method for detecting myoglobin, and relates to the technical field of antibodies. The antibody or a functional fragment thereof disclosed by the invention comprises a heavy chain complementarity determining region and a light chain complementarity determining region. The antibody or the functional fragment thereof has binding capacity and good affinity to myoglobin; and the specificity and sensitivity of myoglobin detection by using the antibody or the functional fragment thereof are good.

Description

technical field [0001] The present invention relates to the technical field of antibodies, in particular to an anti-myoglobin antibody, a kit and a method for detecting myoglobin. Background technique [0002] Myoglobin (myoglobin globin, Mb) is a protein that stores and distributes oxygen in mammalian cells (mainly muscle cells), and its oxygen saturation curve is hyperbolic. It is composed of a polypeptide chain and a prosthetic heme, with a relative molecular mass of 16700 and 153 amino acid residues. The apomyoglobin without heme is called globin (globin), which has obvious homology with the subunits of hemoglobin (a-globin chain and p-globin chain) in amino acid sequence, and their conformation and The functions are also very similar. [0003] Most of the hydrophobic side chains on the amino acid residues in the polypeptide chain of myoglobin are inside the molecule, and the hydrophilic side chains are mostly located on the surface of the molecule, forming a compact s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K16/18C12N15/85C12N5/10G01N33/68G01N33/577
CPCC07K16/18C12N15/85G01N33/6893G01N33/6887G01N33/577C07K2317/565C07K2317/92C07K2317/567G01N2333/805G01N2800/324G01N2800/32
Inventor 崔鹏何志强孟媛钟冬梅叶颖覃婷王晨
Owner DONGGUAN PENGZHI BIOTECH CO LTD