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Application of reagent for detecting gene methylation and colorectal cancer diagnosis or auxiliary diagnosis kit

A technology for auxiliary diagnosis and colorectal cancer, applied in the field of tumor diagnosis, which can solve the problems of unsatisfactory marker gene detection effect and other problems

Active Publication Date: 2022-01-14
WUHAN AIMISEN LIFE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there are not many methylation markers (markers) that can be used to detect colorectal cancer, and the detection effect of some marker genes is not satisfactory. Considering the huge number of patients with colorectal cancer, it is necessary to screen more and better colorectal cancer Cancer detection marker or marker combination is very necessary

Method used

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  • Application of reagent for detecting gene methylation and colorectal cancer diagnosis or auxiliary diagnosis kit
  • Application of reagent for detecting gene methylation and colorectal cancer diagnosis or auxiliary diagnosis kit
  • Application of reagent for detecting gene methylation and colorectal cancer diagnosis or auxiliary diagnosis kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This embodiment provides a method for diagnosis or auxiliary diagnosis of colorectal cancer, which includes:

[0059] Use the methylation-specific PCR method to detect the methylation of any of the following genes in the sample:

[0060] ADAMTS1, AKR1B1, ARHGAP20, BEND5, BVES, C17orf102, CHRDL1, DMRT1, DPY19L2, EPHA7, FAM19A5, FAM150A, FAM184B, FBXL21, GDF6, GLB1L3, GPR27, GRIN2A, HBM, INA, KCNA1, LAYN, NPTXL, RSPO3, SLC35F1, SLITRK5, SNAP91, SNX32, SPG20, SPSB4, STK32B, T, THSD7B, TMEM130, TMEM132E, TRHDE, TRIM71, TRPC6, UNC5C, VAT1L, VWC2, WDR17, WT1, ZNF43, ZNF492, ZNF625, ZNF7943B, ZNF8 ZNF829 and ZNF880.

[0061] Specific steps include:

[0062] (1) DNA extraction

[0063] Use a DNA extraction kit to extract the DNA of the sample to be tested, for example, UnigeneDx from Ningbo Youcheng Biomedical Technology Co., Ltd. For specific experimental operations, refer to the manufacturer's instructions.

[0064] (2) Sulfite conversion

[0065] The nucleic acid conver...

experiment example 1

[0082] Select 300 cases of colorectal cancer tissue samples and 90 cases of normal intestinal tissue samples, use the method in Example 1 to diagnose colorectal cancer, output the Ct value of each detection gene in each sample, and use R to make ROC curve for these Ct values, The Ct value at the maximum Youden index was used as the cut-off value to obtain the sensitivity, specificity and AUC value. The results are shown in Table 3 and figure 1 .

[0083] table 3

[0084]

[0085]

[0086]It can be seen that the sensitivity of these 52 marker genes is between 59.3% and 97.7%, among which there are 31 marker genes with a sensitivity above 90%, namely: ADAMTS1, ARHGAP20, BEND5, BVES, DMRT1, DPY19L2, FAM19A5, FAM184B , FBXL21, GDF6, GLB1L3, GRIN2A, INA, KCNA1, LAYN, NPTX1, PXDN, RELN, SLC35F1, SNAP91, SPG20, STK32B, TMEM130, TMEM132E, TRHDE, TRPC6, UNC5C, VWC2, WT1, ZNF625 and ZNF804B, each marker gene The specificity of the genes was high, ranging from 93.3% to 100%, and ...

experiment example 2

[0088] Using the same sample as in Experimental Example 1, the method of Example 1 was used to diagnose colorectal cancer with the combination of two genes. The results are shown in Table 4 below.

[0089] Table 4

[0090]

[0091]

[0092] It can be seen that using the gene combinations in Table 2 for diagnosis, the sensitivity is above 90%, the specificity is 100%, and the AUC reaches 0.976.

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Abstract

The invention discloses application of a reagent for detecting gene methylation and a colorectal cancer diagnosis or auxiliary diagnosis kit, and relates to the technical field of tumor diagnosis. The invention discloses methylation of the following genes as markers for diagnosis of colorectal cancer: ARHGAP20, BEND5, DMRT1, DPY19L2, FBXL21, GDF6, GLB1L3, GRIN2A, INA, KCNA1, LAYN, NPTX1, PXDN, RELN, SLC35F1, SNAP91, STK32B, TMEM130, TMEM132E, TRHDE, TRPC6, UNC5C, VWC2, WT1, ZNF625 and the like. The invention provides a new idea for diagnosis of colorectal cancer.

Description

technical field [0001] The invention relates to the technical field of tumor diagnosis, in particular to the application of a reagent for detecting gene methylation and a diagnostic or auxiliary diagnostic kit for colorectal cancer. Background technique [0002] Colorectal cancer is the most common malignant tumor in my country and even in the world. Statistics in 2018 show (Bray, F., et al., Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries.CA Cancer J Clin, 2018.68(6):p.394-424), its morbidity and mortality rank third among all malignant tumors. The current gold standard for the diagnosis of colorectal cancer is colonoscopy, but this procedure is invasive and expensive, so it is not suitable as a basis for large-scale screening and early diagnosis. [0003] Most colorectal tumors grow slowly, remain silent, or are asymptomatic until they reach a certain size or stage of development, during which a series ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/154
Inventor 张良禄李婷婷万康康吴志诚
Owner WUHAN AIMISEN LIFE TECH CO LTD
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