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Lactobacillus paracasei CCFM1229 for relieving depressive mood and application thereof

A CCFM1229, Lactobacillus technology, applied in the field of microorganisms, can solve the problems of large side effects, slow onset, inconsistent treatment effects, etc., and achieve the effect of reducing activity, maintaining stability, and restoring intestinal flora disorder.

Pending Publication Date: 2022-07-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently widely used antidepressant drugs in clinical treatment, such as Selective Serotonin Reuptake Inhibitor (SSRI), generally have the disadvantages of slow onset of action, inconsistent therapeutic effects, and large side effects, making the treatment of depression difficult. has serious limitations

Method used

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  • Lactobacillus paracasei CCFM1229 for relieving depressive mood and application thereof
  • Lactobacillus paracasei CCFM1229 for relieving depressive mood and application thereof
  • Lactobacillus paracasei CCFM1229 for relieving depressive mood and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0112] Example 1: Acquisition and identification of Lactobacillus paracasei CCFM1229

[0113] 1. Isolation and screening of Lactobacillus paracasei:

[0114] (1) Take 1 mL of Chongqing pickle water, apply it to MRS solid medium after gradient dilution, and place it in an anaerobic environment at 37° C. for 72 hours; observe and record the colony morphology, pick and purify the colony by streaking; in MRS liquid medium , cultured at 37°C for 48 hours, the resulting colonies were Gram-stained, and the colony morphology was recorded. Gram-negative bacterial strains and Gram-positive cocci in the colony were discarded, and Gram-positive bacilli were selected.

[0115] (2) After the catalase analysis, the catalase-positive strains were discarded, and the catalase-negative strains were retained.

[0116] 2. Molecular biological identification of Lactobacillus paracasei:

[0117] (1) Single bacterial genome extraction: The catalase-negative strain screened in step 1 was cultured o...

Embodiment 2

[0137] Example 2: Lactobacillus paracasei CCFM1229 reduces depression-like behavior in mice

[0138] Specific steps are as follows:

[0139] (1) Lactic acid bacteria gavage:

[0140] Activation of strains:

[0141] Lactobacillus paracasei CCFM1229, Lactobacillus paracasei 29L1, and Lactobacillus paracasei ATCC25302 stored in the respective bacterial banks were streaked on MRS plates, and after culturing at 37 °C for 48 hours, a single colony was picked from the plate and inoculated to the corresponding 5mL In the MRS liquid medium, after culturing at 37°C for 24 hours, inoculate the corresponding fresh MRS liquid medium at a ratio of 2% (v / v), and culture at 37°C for 24 hours to prepare Lactobacillus paracasei CCFM1229 seeds. liquid, Lactobacillus paracasei 29L1 seed liquid, Lactobacillus paracasei ATCC25302 seed liquid.

[0142] Preparation of bacterial suspension:

[0143] Get respectively the Lactobacillus paracasei CCFM1229, Lactobacillus paracasei 29L1 of above-mentio...

Embodiment 3

[0160] Example 3: Lactobacillus paracasei CCFM1229 can significantly increase the level of neurotransmitter (5-HT) in the brain of depressed mice

[0161] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The same as in Example 2, the mice in Example 2 were euthanized at the end of the eighth week, and the mouse brain tissue was collected, and the prefrontal lobes were isolated on ice.

[0162] Take a certain quality of fresh prefrontal cortex, add 30 times the volume of 0.2M perchloric acid solution (equivalent to 1 g tissue plus 30 mL of homogenate perchloric acid solution), use a tissue homogenizer to homogenize, and the tissue fluid passes through 12000g After 10 min of centrifugation, the supernatant was drawn and filtered through a 0.22 μm water-based filter, and the content of 5-HT was detected by HPLC.

[0163] The experimental results are as image 3 As shown in A, the content of 5-HT in the normal control group, depression model group, CCFM1229 intervention group, 2...

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Abstract

The invention discloses lactobacillus paracasei CCFM1229 for relieving depressive mood and application of the lactobacillus paracasei CCFM1229, and belongs to the technical field of microorganisms. The lactobacillus paracasei can relieve depression-like behaviors of depression mice, increase the content of neurotransmitter 5-hydroxytryptamine in the brain, increase the level of BDNF in hippocampus of the depression mice, inhibit expression of Crh mRNA of hypothalamus and reduce the level of corticosterone in serum; the lactobacillus paracasei can reduce the activity of cerebral cortex xanthine oxidase, improve the activity of liver adenosine deaminase and reduce the content of serum uric acid; the prefrontal lobe Mbp mRNA level is improved, and the stability of the CNS myelin sheath structure and function is maintained; the mRNA levels of prefrontal lobes Grin1, Grin2a and Grin2b can be improved, and the method has very important significance on synaptic plasticity; the lactobacillus paracasei can improve the diversity of intestinal flora and degrade omethoate, and has a wide application prospect.

Description

technical field [0001] The invention relates to Lactobacillus paracasei CCFM1229 for relieving depression and application thereof, belonging to the technical field of microorganisms. Background technique [0002] Depression (Major Depressive Disorder, MDD) is a common mood disorder. Currently, more than 350 million people worldwide suffer from depression, and the incidence has continued to rise in recent years. Depressed patients are persistently depressed, pessimistic and misanthropic, their cognition is deviated from that of healthy people, they suffer from mental pain, and in severe cases are even accompanied by physical symptoms. Depression is an important public health problem and one of the leading causes of the global disease burden. However, currently widely used antidepressant drugs in clinical treatment, such as Selective Serotonin Reuptake Inhibitor (SSRI), generally have disadvantages such as slow onset of action, inconsistent treatment effect, and large side ef...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L2/38A23L29/00A23L33/135A61K35/747A61P25/24A61P29/00A62D3/02C12R1/225A62D101/04A62D101/28
CPCC12N1/20A61K35/747A61P25/24A61P29/00A23L33/135A23L29/065A23L2/382A62D3/02A23V2002/00A62D2101/04A62D2101/28A23V2400/165A23V2200/322A23V2200/30A23V2200/3204Y02A50/30
Inventor 王刚许梦舒田培郡赵建新张灏陈卫
Owner JIANGNAN UNIV
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