Transplanting body for constructing bile duct cancer xenotransplantation model and preparation method and application of transplanting body

Abstract

The invention belongs to the technical field of biological medicine, and discloses a transplanting body for constructing a bile duct cancer xenotransplantation model and a preparation method and application of the transplanting body. The preparation method comprises the steps that a culture base body is prepared; bile duct cancer cells are separated, specifically, solid tumor tissues of bile duct cancer are dissociated by using dissociation liquid to obtain the bile duct cancer cells; the bile duct cancer cells obtained by dissociation are planted on the polarized surface of the carbon nanostructure of the culture base body; and the bile duct cancer cells are cultured on the culture base body until the bile duct cancer tissues are formed, and the transplanting body for constructing the bile duct cancer xenotransplantation model is obtained. According to the preparation method, the transplantation body containing the bile duct cancer organoid is obtained by utilizing the cancer cells, when the transplantation body is utilized to construct the bile duct cancer xenotransplantation model, a relatively high success rate can be obtained under the condition of a small amount of tissues, and the time required for constructing the xenotransplantation model is relatively short.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a transplant body for constructing a cholangiocarcinoma xenograft model, a preparation method and use thereof. Background technique [0002] Currently, cholangiocarcinoma refers to malignant tumors originating from the extrahepatic bile duct, including the bile duct from the hilar region to the lower end of the common bile duct. Its etiology may be related to diseases such as bile duct stones and primary sclerosing cholangitis. Surgical treatment, radiotherapy, chemotherapy and other methods can be used clinically, but the effect is not obvious and the prognosis is poor. In order to improve the therapeutic effect of cholangiocarcinoma, in-depth research on the pathogenic mechanism and drug resistance of cholangiocarcinoma is needed, and the animal xenograft model is an important tool for research. [0003] In related technologies, the primary or metastatic tumor...

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Problems solved by technology

[0003] In related technologies, the primary or metastatic tumor tissue blocks of patients with cholangiocarcinoma are mainly used as transplants. However, the number of primary or metastatic tumor tissue blocks in patients with cholangiocarcinoma is small, resulting in insufficient number of transplants, and the acquisition method is cumbersome. Painful, low success rate and long time required to construct cholangiocarcinoma xenograft model

[0004] Through the above analysis, the existing problems and defects of the existing technology are: the number of primary or metastatic tumor tissue blocks of patients with cholangiocarcinoma is insufficient as transplants, and the acquisition method is cumbersome, and the patient suffers a lot. To construct a xenograft model of cholangiocarcinoma has a lower success rate and takes longer

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