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Mycobacterium tuberculosis detection method and detection kit

A technology of Mycobacterium tuberculosis, detection kit, applied in the directions of microorganism-based methods, microbial assay/inspection, biochemical equipment and methods, etc., can solve problems such as false positive PCR results

Pending Publication Date: 2022-01-21
DELTA ELECTRONICS INC
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Problems solved by technology

In addition, some studies have pointed out that several inhibitors in sputum may affect nucleic acid amplification and cause false negative NAAT results, which rarely occurs in AFS positive samples
Also, some occasional or systematic errors (eg, primers / probes cross-reactive with NTM or other bacteria, viruses, and fungi) may also lead to false positive PCR results

Method used

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  • Mycobacterium tuberculosis detection method and detection kit
  • Mycobacterium tuberculosis detection method and detection kit
  • Mycobacterium tuberculosis detection method and detection kit

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Embodiment Construction

[0023] Some embodiments embodying the features and advantages of the present application will be described in detail in the description in the following paragraphs. It should be understood that the application can have various changes in different aspects without departing from the scope of the application, and that the description and drawings therein are illustrative in nature and not intended to limit the application.

[0024] The present application provides a detection method and a detection kit for Mycobacterium tuberculosis performed by single-tube nested quantitative PCR (one-tube nested quantitative PCR). Nested qPCR mainly involves the use of two primer pairs and a two-step process, first with a first round of amplification by outer primers, followed by a second round of amplification with inner primers. Compared with traditional qPCR (or real-time PCR), nested qPCR performs two PCR reactions in the same reaction tube; the first round of PCR amplification is carried ...

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Abstract

A Mycobacterium tuberculosis detection method includes performing nested qPCR analysis on a sample, wherein sequences of a pair of external primer used in a first round of amplification are as shown in sequence numbers 1 and 2, and sequences of a pair of internal primers and a probe used in a second round of amplification are as shown in sequence numbers 3 to 5.

Description

technical field [0001] The application relates to a detection method and a detection kit for germs, especially a detection method and a detection kit for Mycobacterium tuberculosis. Background technique [0002] Tuberculosis (TB for short) is an infectious disease caused by Mycobacterium tuberculosis (MTB for short). Tuberculosis is one of the most important infectious diseases globally, killing 1.5 million people every year. To reduce morbidity and mortality and prevent the spread of tuberculosis, accurate and rapid early diagnosis is especially important. [0003] The traditional tuberculosis diagnosis mainly relies on the detection of acid fast bacilli (AFB) by smear microscope, and then cultures Mycobacterium tuberculosis on a selective medium. Although acid-fast staining (AFS) is an economical, rapid and simple method, and the overall detection sensitivity can reach 71.4% in lung samples, it drops to 24% in extrapulmonary samples, which is mainly The reason is that t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/6848C12Q1/6851C12Q1/06C12R1/32
CPCC12Q1/689C12Q1/6848C12Q1/6851C12Q2531/113C12Q2549/119C12Q2549/113C12Q2547/101C12Q1/686
Inventor 李怡臻邹志成吴旻宪王信尧林倩如
Owner DELTA ELECTRONICS INC
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