Magnetic nano composite material as well as preparation method and application thereof

A composite material and magnetic nanotechnology, applied in biochemical equipment and methods, material analysis and material analysis through electromagnetic means, can solve the problems of complicated operation steps and long time consumption, and achieve simple operation, uniform distribution and high detection rate fast effect

Pending Publication Date: 2022-02-18
CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhang Min prepared Fe 3 o 4 MNPs are used for DNA adsorption, the reaction temperature is 200°C, and the reaction time is as long as 48h. The method for preparing MNPs in the prior art is complicated and time-consuming

Method used

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  • Magnetic nano composite material as well as preparation method and application thereof
  • Magnetic nano composite material as well as preparation method and application thereof
  • Magnetic nano composite material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] The preparation of embodiment 1 magnetic nanocomposite material

[0104] First, a hole was opened at 45 ml of the centrifuge tube (50 ml, Corning), and the nitrogen blowing needle hole was inserted downward into the hole. Weigh FeCl 3 ·6H 2 O 0.448g and FeCl 2 4H 2 O 0.163g, dissolved in 10mL of deionized water and 10mL of ethanol (1:1), completely dissolved and transferred to a centrifuge tube. Insert the nitrogen blowpipe below the liquid level and keep filling with nitrogen for 20 minutes to remove the oxygen in the system. Immediately after deoxygenation, add 1 ml of ammonia water to the system for an alkaline environment, unplug the nitrogen blowpipe and tighten the bottle cap, and put the centrifuge tube into a multi-tube vortex shaker for 10 minutes. At this time, the nitrogen blowing needle continues to pass nitrogen to maintain the nitrogen content in the system. After the reaction is over, transfer the product to a complete centrifuge tube, centrifuge at...

Embodiment 2

[0106] Embodiment 2 magnetic nano composite material B

[0107] In this embodiment, the content of ethyl orthosilicate added is 0.5 mL, and other reaction steps and conditions are the same as in Embodiment 1.

Embodiment 3

[0108] Embodiment 3 magnetic nanocomposite material C

[0109] In this embodiment, the content of ethyl orthosilicate added is 1.5 mL, and other reaction steps and conditions are the same as in Embodiment 1.

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Abstract

The invention discloses a magnetic nano composite material and a preparation method and application thereof. The magnetic nano composite material is of a core-shell structure, an inner core is Fe3O4, a shell layer is SiO2, and a middle layer is further arranged between the inner core and the shell layer; wherein the intermediate layer is from a polymer; and the polymer is selected from at least one of polyvinylpyrrolidone and polydopamine. According to the magnetic nano composite material provided by the invention, sample DNA can be rapidly extracted, and the extracted DNA is applied to a qPCR (real-time fluorescence quantification) detection technology.

Description

technical field [0001] The invention relates to a magnetic nanocomposite material and its preparation method and application, belonging to the technical field of magnetic nanomaterials. Background technique [0002] Vibrio parahaemolyticus (VP) is a Gram-negative halophilic bacterium that is commonly found in seawater environments and seafood such as fish, shrimp, crab, and shellfish. If humans eat VP-contaminated food by mistake, it will cause food poisoning, gastrointestinal discomfort, vomiting, diarrhea, fever and other symptoms, and even life-threatening in severe cases; VP will cause vibriosis after infecting marine organisms, which will cause serious damage to the aquaculture industry. Cause huge economic losses, VP has caused serious damage and threat to human health and safety and property safety. Food poisoning caused by VP has a high incidence and a wide distribution range, and is a public safety issue that is highly valued both at home and abroad. In my country...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30G01N27/447G01N21/64C12Q1/6806C12Q1/689C12Q1/06C12R1/63
CPCB01J20/103B01J20/06B01J20/261B01J20/262B01J20/28009B01J20/28004C12Q1/6806C12Q1/689G01N27/447G01N21/6428B01J2220/4806B01J2220/4812B01J2220/46C12Q2523/308C12Q2521/327
Inventor 吴立冬肖雨诗曹强刘欢刘娜孙蒙蒙李佩仪
Owner CHINESE ACAD OF FISHERY SCI
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