Method for researching paulownia arbuscular disease based on m6A methylation sequencing

Abstract

The invention discloses a method for researching paulownia arbuscular disease based on m6A methylation sequencing, and belongs to the field of molecular biology. Comprising the following steps: (1) extracting total RNA (Ribonucleic Acid) of paulownia; (2) m6A sequencing: constructing an m6A library by using the paulownia total RNA, and sequencing; (3) transcriptome sequencing: constructing a transcriptome library by using the paulownia total RNA, and sequencing; (4) bioinformatics analysis: performing Peak analysis on an m6A sequencing result, drawing a paulownia fortunei m6A modification map, and performing m6Amotif prediction on the basis of a Peak analysis result; performing transcriptome analysis on a transcriptome sequencing result; and carrying out correlation analysis on the m6A and the transcriptome, and screening out the paulownia arbuscular disease related gene. The paulownia arbuscular disease related genes are analyzed and screened through the method, a research thought is provided for further exploring the pathogenesis of the paulownia arbuscular disease, a foundation is laid for further exploring the pathogenesis of the paulownia arbuscular disease, and prevention and control research on the paulownia arbuscular disease is facilitated.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a method for studying Paulownia arbuscular disease based on m6A methylation sequencing. Background technique [0002] Paulownia, bark gray, taupe or gray black, smooth when young, longitudinally fissured when old; false bifurcated branches, simple leaves, opposite, large leaves, ovate, entire or lobed, with long stalks, There are fluff on the stalk; it likes light, is more shade-tolerant, likes warm climates, and is not cold-resistant; it grows fast, tolerates barrenness, and has excellent material properties. It plays an important role in wind and sand fixation, ensuring food security and timber supply. [0003] In production, the Paulownia arbuscular disease caused by phytoplasma is an important factor restricting the development of Paulownia industry, because the Paulownia arbuscular phytoplasma cannot be cultured in vitro, which seriously limits the eluc...

AI Technical Summary

Problems solved by technology

[0003] In production, Paulownia arbuscular disease caused by phytoplasma is an important factor restricting the development of Paulownia industry, because Paulownia arbuscular phytoplasma cannot be cultured in vitro, which seriously limits the elucidation of the pathogenesis of Paulownia arbuscular disease

The occurrence and development of Paulownia arbuscular disease is a very complicated process. At present, the pathogenesis of Paulownia arbuscular disease has not yet been elucidated, and effective methods for the prevention and treatment of arbuscular disease have not yet been established. Therefore, it is necessary to open up new ways for research

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