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Urine guanine nucleotide binding protein subunit alpha-13 and application of polypeptide fragment of urine guanine nucleotide binding protein subunit alpha-13 in burns

A technology of guanine nucleotide and binding protein, which is applied in the field of urine guanine nucleotide binding protein subunit α-13 and its polypeptide fragments, which can solve the problems of poor skin integrity and aggravate the pain of patients, and achieve convenient preservation Effect

Pending Publication Date: 2022-03-01
张曼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the integrity of the skin of patients with severe burns is poor. As an invasive skin test, there are many difficulties in the clinical application of blood tests for such patients. Repeated blood testing will also aggravate the suffering of patients.

Method used

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  • Urine guanine nucleotide binding protein subunit alpha-13 and application of polypeptide fragment of urine guanine nucleotide binding protein subunit alpha-13 in burns
  • Urine guanine nucleotide binding protein subunit alpha-13 and application of polypeptide fragment of urine guanine nucleotide binding protein subunit alpha-13 in burns

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Urine Specimen Collection and Processing

[0021] The burn patients were selected as the burn group, and the healthy subjects who underwent physical examination during the same period were selected as the normal control group. Collect 30ml of fresh morning urine samples from the research subjects in each group after admission, and collect the urine in the catheter in the morning for those who cannot urinate normally, and place them in a dry and clean container. After the collected urine samples were centrifuged at 4000r / min for 5min, the supernatant was aspirated, divided into 2ml tubes, and stored in a -80°C refrigerator.

Embodiment 2

[0022] Example 2 Mass Spectrometry and Screening of Urine Peptides

[0023] Extract protein from urine samples and determine the concentration of the extracted protein. Mass spectrometric analysis of urine samples was performed by an OrbitrapFusion type mass spectrometer. Quantitative calculation was performed on the data obtained in the mass spectrometer of the experimental group and the normal control group. The difference between groups was analyzed by t-test, and the difference in protein expression was more than 1.5 times and the statistical test P<0.05 was used as the reference standard to screen for differentially expressed proteins.

Embodiment 3

[0024] Example 3 Identification and Analysis of Differential Peptides

[0025] The database used was the Uniprot_Homo database, and the original mass spectrum files were processed by MaxQuant software. The search parameter settings are shown in Table 1.

[0026]

[0027] Compared with healthy people, guanine nucleotide binding protein subunit α-13 was lower expressed in the urine of burn patients, and its content in the urine of healthy control group and burn group was as follows: figure 1 As shown, the expression of guanine nucleotide binding protein subunit α-13 in the urine of the normal control group and the burn group had significant differences, and decreased with the aggravation of the burn degree.

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Abstract

The invention provides a urine guanine nucleotide binding protein subunit alpha-13 (guanine nucleotide binding protein subunit alpha-13) and an application of a polypeptide fragment of the urine guanine nucleotide binding protein subunit alpha-13. The invention further provides a preparation method of the urine guanine nucleotide binding protein subunit alpha-13 and an application of the polypeptide fragment of the urine guanine nucleotide binding protein subunit alpha-13. The invention particularly relates to application of a urine guanine nucleotide binding protein subunit alpha-13 and a polypeptide fragment thereof in preparation of preparations for burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like. Burns are common important wounds in daily life, about 5000-100000 people in every one million people are burnt every year, according to statistics of World Health Organization, more than 300,000 people die from burn patients globally every year, and the treatment survival rate of serious burns is still at a lower level. Researches prove that compared with healthy people (normal control groups), the guanine nucleotide binding protein subunit alpha-13 and the polypeptide fragments thereof in the urine have reduced expression in burn patients, and the content of the guanine nucleotide binding protein subunit alpha-13 and the polypeptide fragments thereof is gradually reduced along with the aggravation of the burn degree. The method can be used for various purposes of application detection of burn patients. According to the invention, the advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of a urine sample are exerted, and the urine sample is used for detecting the guanine nucleotide binding protein subunit alpha-13 and the polypeptide fragment thereof.

Description

technical field [0001] The present invention relates to new uses of urinary guanine nucleotide binding protein subunit α-13 and its polypeptide fragments, in particular to the use of urinary guanine nucleotide binding protein subunit α-13 and its polypeptide fragments in the diagnosis and identification of burns Application of diagnosis, evaluation of burn area and degree, evaluation of treatment effect, monitoring, prognosis evaluation and mechanism research, etc. Background technique [0002] Burn refers to the damage of human skin, tissues and even deep organs caused by chemical substances such as flame, high temperature gas, hot solid or liquid, radiation, electric energy, strong acid and strong alkali, and is a systemic comprehensive disease. Massive necrosis of wound tissue, infection, shock, and coagulation dysfunction after burns can cause a series of pathophysiological changes in the body. Different degrees of burns have different effects on the human body. Severe ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/545
CPCG01N33/6893G01N33/6848G01N33/545G01N2333/47G01N2800/40G01N2800/52
Inventor 张曼王佶图
Owner 张曼