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Fingerprint detection method and application of traditional Chinese medicine compound cubeba and huizhou Pingwei granules

A technology of fingerprint spectrum and detection method, applied in the field of fingerprint spectrum detection, can solve the problem of lack of fast, simple and accurate analysis methods, etc., and achieve the effect of improving the quality evaluation system, being beneficial to quality monitoring and simple sample pretreatment.

Pending Publication Date: 2022-03-11
合肥创新医药技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the composition analysis, quality control and pharmacology of Canghuopingwei Granules, Xu Feifei has reported the research on Canghuopingwei Granules (2012, Master Thesis of Hefei University of Technology). Research on the therapeutic effect of Wei Granules on chronic gastritis and bile reflux gastritis in rats (Chinese Materia Medica, 2012, 35(07): 1123-1125), that is to say, the current Chinese medicine compound and similar compositions of Canghuo Pingwei Granules The research on the fingerprints of Canghuo Pingwei Granules has not been reported in the literature, and there is no fast, simple and accurate analysis method suitable for the analysis and detection of various chemical components in Canghuopingwei Granules, including the identification of characteristic components and content distribution and other related information

Method used

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  • Fingerprint detection method and application of traditional Chinese medicine compound cubeba and huizhou Pingwei granules
  • Fingerprint detection method and application of traditional Chinese medicine compound cubeba and huizhou Pingwei granules
  • Fingerprint detection method and application of traditional Chinese medicine compound cubeba and huizhou Pingwei granules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Preparation of Canghuo Pingwei Granules ,Specific steps are as follows:

[0049] ① Prepare raw materials according to the following parts by weight: fried atractylodes 333, Huoxiang leaves 278, dandelion 694, ginger officinalis 278, tangerine peel 278, red peony root 555, Poria cocos 278, Jiang Pinellia 126, Gansong 179, sea octopus 333, white and 416, Licorice 139, Adenophora 162, Ophiopogon 198;

[0050] ② Take Magnolia officinalis and use 70% ethanol solution to reflux extract 3 times, add 11 times the amount of 70% ethanol for the first time, add 10 times the amount of 70% ethanol for the second time, extract for 2 hours each time, and filter;

[0051] ③ reclaiming ethanol after merging the extracts in step ②, and concentrating into an extract whose relative density is 1.05-1.10;

[0052] ④Soak Atractylodes atractylodis, Huoxiang and tangerine peel in 10 times the amount of water for 1 hour, extract the volatile oil by steam distillation for 5 hours, filter, ...

Embodiment 2

[0069] Example 2 Methodological Verification of Canghuopingwei Granule Fingerprint

[0070] (1) Specificity test

[0071] Get each 10 μ L of the blank solvent methanol, the mixed reference substance solution for positioning in the embodiment 1, and the test solution for detection in the embodiment 1, and use the high-performance liquid chromatography conditions in the embodiment 1 to test, and record the collection of spectra. The results are shown in Figure 4 (a), Figure 4 (b) and Figure 4 (c), it can be seen that the blank solvent methanol does not interfere with the determination, which shows that the fingerprint detection method of Canghuopingwei granules in the present invention has good specificity.

[0072] (2) Precision test

[0073] Get the need testing solution for detection in embodiment 1, adopt the high performance liquid chromatography condition identical with embodiment 1, repeat sample injection 6 times, measure the relative retention time, relative p...

Embodiment 3

[0096] This embodiment adopts the same implementation as in Example 1, the difference is that the high performance liquid chromatography conditions are: the chromatographic column is an Agilent 5HC-C18 (4.6×200mm, 5 μm) chromatographic column, and the mobile phase A It is acetonitrile, the mobile phase B is 0.15% phosphoric acid aqueous solution by volume fraction, the detection wavelength is 203nm, the flow rate is 1.0mL / min, the column temperature is 35°C, and the injection volume is 10μL.

[0097] Test results such as Image 6 as shown in the Image 6 It can be seen that slightly changing the concentration of phosphoric acid aqueous solution has no obvious influence on the detection results.

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Abstract

The invention discloses a detection method and application of a fingerprint spectrum of traditional Chinese medicine compound cubeba and huizhou Pingwei granules. The detection method is established by adopting a high performance liquid chromatography, and a chromatographic column adopts an octadecyl silane bonded silica gel chromatographic column; a DAD detector is adopted as the detector; a mobile phase A is acetonitrile or methanol, a mobile phase B is a formic acid aqueous solution, an acetic acid aqueous solution or a phosphoric acid aqueous solution, and gradient elution is carried out according to a specific program, so that related information such as the number of chemical components, the positions of characteristic components, content distribution and the like of the traditional Chinese medicine compound cumjopin particles can be rapidly, simply, comprehensively, accurately and reliably detected. Therefore, the quality evaluation system of the preparation is improved, the quality general picture of the preparation is objectively reflected, production and quality detection are guided, and the clinical effectiveness and safety of the preparation are ensured; meanwhile, a scientific basis is provided for research on a quality marker, a pharmacodynamic material basis and an action mechanism of the compound preparation.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine analysis, and in particular relates to a fingerprint detection method and application of traditional Chinese medicine compound Canghuopingwei granules. Background technique [0002] Canghuo Pingwei Granules is an innovative traditional Chinese medicine. The prescription comes from the clinical experience of Professor Zhang Qionglin, a famous old Chinese doctor in Anhui Province, who has been practicing medicine for more than 60 years - "Jiawei Pingwei San", "Pingwei San" in Song "Taiping Huimin Heji Bureau Prescription" It has been used for spleen and stomach diseases for more than 900 years. It is composed of atractylodes atractylodes, Magnolia officinalis, tangerine peel and licorice. In more than 60 years of clinical practice, Professor Zhang has added, subtracted, cut, repeatedly refined and screened Canghuoping on the basis of "Pingweisan" prescription. The prescription o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/86G01N30/62G01N30/34
CPCG01N30/02G01N30/8686G01N30/62G01N30/34Y02A50/30
Inventor 董宠叶立仁许龙黄美容杨转转曹阳黄顺旺曹明成
Owner 合肥创新医药技术有限公司
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