High performance liquid chromatography detection method for gingerol component content

A high-performance liquid phase and detection method technology, applied in the field of analytical chemistry, can solve the problems of cumbersome operation and low accuracy, and achieve the effects of sensitive response, high accuracy and easy process

Pending Publication Date: 2022-03-18
泉州海关综合技术服务中心
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a high-efficiency liquid phase detection method...

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A high-efficiency liquid phase detection method for gingerol content, comprising the following steps:

[0021] 1) Take the sample to be tested and dilute it to 0.6-80mg / mL with 80% methanol solution as the solution to be tested; take another standard gingerol and add 80% methanol solution to dilute it into several reference solutions with known concentrations ;

[0022] 2) Perform detection with the following chromatographic conditions: the filler is amylose-three (3-chloro-5-methylphenylcarbamate); mobile phase A: ethanol-phosphate solution, wherein the volumetric concentration of ethanol is 40% to 50%, the phosphate solution also contains 0.2mol / L of KH 2 PO 3 and a K of 0.36mol / L 2 HPO 3 ; Mobile phase B: acetonitrile; the volume ratio of mobile phase A to mobile phase B is 30:40~20:70; detector: evaporative light scattering detector; column temperature: 34~38°C; detection wavelength: 330~350nm; Flow rate: 0.3~0.6mL / min;

[0023] 3) Take 30 μL of the solution t...

Embodiment 2

[0025] A high-efficiency liquid phase detection method for gingerol content, comprising the following steps:

[0026] 1) Take the sample to be tested and dilute it to 0.6-80mg / mL with 80% methanol solution as the solution to be tested; take another standard gingerol and add 80% methanol solution to dilute it into several reference solutions with known concentrations ;

[0027] 2) Perform detection with the following chromatographic conditions: the filler is amylose-three (3-chloro-5-methylphenylcarbamate); mobile phase A: ethanol-phosphate solution, wherein the volumetric concentration of ethanol is 40% to 50%, the phosphate solution also contains 0.2mol / L of KH 2 PO 3 and a K of 0.36mol / L 2 HPO 3 ; Mobile phase B: acetonitrile; the volume ratio of mobile phase A to mobile phase B is 30:40~20:70; detector: evaporative light scattering detector; column temperature: 34~38°C; detection wavelength: 330~350nm; Flow rate: 0.3~0.6mL / min;

[0028] 3) Take 30 μL of the solution t...

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Abstract

The invention provides a high performance liquid detection method for the content of gingerol components. According to the technical scheme, firstly, response characteristics of gingerol components in HPLC detection are analyzed, and a specific detection method is designed on the basis. Specifically, the method comprises the following steps: respectively preparing a to-be-detected sample and a reference substance solution by using a methanol solution, carrying out column chromatography by using amylose-tri (3-chloro-5-methyl phenyl carbamate) as a filler on the basis, and adopting an ethanol-phosphate solution and acetonitrile as mobile phases under the conditions that the column temperature is 34-38 DEG C, the detection wavelength is 330-350 nm, the flow rate is 0.3-0.6 mL/min and the sample size is 30 [mu] L; linear gradient elution is adopted, and the content is calculated according to the peak area by adopting an internal standard method after detection. The method is simple and convenient to operate, the detection efficiency is remarkably improved, the process is easy to standardize, the response is sensitive, the accuracy is high, and the method has a good popularization prospect and outstanding technical advantages.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a high-efficiency liquid phase detection method for the content of gingerol components. Background technique [0002] Gingerol is a general term for gingerol, gingerol and other spicy substances related to ginger. The research on the components of gingerol has a history of more than 100 years. In 1969, the crystals of gingerol were isolated, and it was confirmed that gingerol is composed of a series of similar compounds. These compounds are [4]-, [6]-, [8]-, [10]-, [12]-gingerol, among which [6]-gingerol has the highest content. So far, the main components of gingerol have been relatively clear. It is a mixture of various substances. The molecular structure of each component substance contains: 3-methoxy-4-hydroxyphenyl functional group. According to the functional group Gingerol can be divided into different types such as gingerol, shogaol, zingerone, zingiberone,...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/74G01N30/86
CPCG01N30/02G01N30/34G01N30/74G01N30/8634G01N2030/045
Inventor 黄伙水翁城武王庆新荣杰峰
Owner 泉州海关综合技术服务中心
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