Miniature single-photon fluorescence microscope implantation device and implantation method

Abstract

The invention provides a miniature single-photon fluorescence microscope implanting device and implanting method, and the device comprises a clamping seat which is provided with a clamping groove; a clamping block; a groove is formed in the bottom face of the clamping groove, a first protrusion is arranged at the end of the clamping block, and a through containing hole is vertically formed in the bottom face of the groove. According to the implanting device, the containing hole is used as a clamping tool of the lens, the lens can be prevented from being damaged and inclined, it can be ensured that the surface of the lens is perpendicular to the bottom face of the clamping groove of the clamping base, the lens leveling process is omitted in the implanting process, and the experiment precision is ensured; the clamping seat and the lens are arranged at the optimal working position at one time as a whole, and all experimental devices are assembled only through one-time operation, so that the influence on animals is reduced; according to the application, the surface of the lens can be parallel to the surface of the lens of the fluorescence microscope without adjusting the lens, and a working distance of 0.48-0.52 mm is reserved, so that the working efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field of neurobiology, in particular to a miniature single-photon fluorescence microscope implantation device and an implantation method. Background technique [0002] At present, in the field of neurobiology, it is possible to inject various neural electrical activity-dependent fluorescent carrier tools into the experimental target brain regions of mice, and observe the intuitive nerve cell activities in deep brain regions with the help of miniature single-photon fluorescence microscopes. During the realization of this technology, it is necessary to complete the assembly of the lens adapted to the microscope and the card slot that fixes the microscope. Among them, because the microscope cannot be directly implanted in the deep brain area, it is necessary to use a special cylindrical lens in conjunction with the microscope as an extension of the lens to capture the field of view in the deep brain area. [0003] A...

AI Technical Summary

Problems solved by technology

Because whether the four walls of the mounting base are perpendicular to the lens surface directly determines whether the surface of the fluorescent microscope lens after entering the mounting base is parallel to the lens surface, so this operation requires sufficient precision, there are great technical problems, and it takes a lot of time.

[0004] In summary, there are following defects in the prior art: 1. Two operations are required, namely, the implantation of the lens for the first time and the installation of the miniature fluorescence microscope mounting seat on the surface of the experimental animal skull for the second time. Mice are anesthetized and fixed, and the two operations will cause additional impact on the animals and cause great interference to the experiment; 2. In the prior art, it is necessary to use a resin frame holder to hold the lens for implantation, which is accompanied by the risk of damaging the lens. The lens is easy to tilt during the clamping process, and it is easy to cause inaccurate implantation coordinates, and the cell activity in the target brain area cannot be observed, resulting in the failure of the experiment; 3. The installation of the fluorescence microscope mount requires sufficient precision, and the operation is time-consuming and laborious.

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