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High-migration-activity UCMSCs, culture method and application of key active components

A culture method and active technology, applied in the field of cells, to achieve the effect of promoting migration

Inactive Publication Date: 2022-04-12
NANJING FANYIDA BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report and application of S-allyl-L-cysteine ​​to improve the migration activity of UCMSCs

Method used

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  • High-migration-activity UCMSCs, culture method and application of key active components
  • High-migration-activity UCMSCs, culture method and application of key active components
  • High-migration-activity UCMSCs, culture method and application of key active components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Test materials

[0022] DMEM / F12 medium and fetal bovine serum were purchased from Gibco. Double antibodies were purchased from Nanjing Senbega Biotechnology Co., Ltd. The PBS buffer was prepared according to the recipe and stored at 4°C, and used up within 24 hours. Type IV collagenase and trypsin were purchased from Shanghai Maokang Biotechnology Co., Ltd. and used according to the instructions. S-allyl-L-cysteine ​​was purchased from source leaf organisms, HPLC≥98%. Cell culture dishes, multi-well plates and Transwell chambers were purchased from Corning. The primary and secondary antibodies of SOX2, NANOG, OCT4 and β-actin were purchased from Beyond Biotech.

[0023] 2. Test method

[0024] 1. Preparation of UCMSCs

[0025] Prepare UCMSCs according to conventional methods, specifically: take about 12 cm of umbilical cord of normal full-term caesarean section neonates (the umbilical cord is stored in PBS buffer containing 1% double antibody at 4°C after coll...

Embodiment 2

[0049] A medium for improving the migration activity of UCMSCs, using DMEM / F12 as a base medium, and adding effective concentration of S-allyl-L-cysteine ​​to the base medium.

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Abstract

The invention discloses UCMSCs with high migration activity, a culture method and application of key active components. The UCMSCs with high migration activity are obtained through culture of a culture medium containing S-allyl-L-cysteine. The invention finds that S-allyl-L-cysteine has the activity of promoting UCMSCs migration and does not influence the dryness of the UCMSCs, so that UCMSCs with enhanced migration activity can be obtained through culture of a culture medium of S-allyl-L-cysteine.

Description

technical field [0001] The invention belongs to the field of cells and relates to stem cell culture, in particular to a UCMSCs with high migration activity, a culture method and the application of key active ingredients. Background technique [0002] Mesenchymal stem cells (MSCs) are derived from the mesoderm in the early stage of development and widely exist in bone marrow, fat, umbilical cord blood and other tissues. They are adult stem cells with self-renewal, high proliferation and multi-directional differentiation potential. MSCs have gradually become an alternative therapy and It is a cell source of great practical value in the field of gene therapy and has broad application prospects in the field of tissue engineering. In recent years, compared with bone marrow MSCs, umbilical cord mesenchymal stem cells (UCMSCs) have attracted more and more attention due to their advantages of easy availability, primitiveness, strong proliferative ability, low immunogenicity, and no ...

Claims

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Application Information

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IPC IPC(8): C12N5/077
Inventor 不公告发明人
Owner NANJING FANYIDA BIOTECHNOLOGY CO LTD