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A soil heavy metal activating bacterial agent and its preparation method and application

A heavy metal and activated bacteria technology, applied in biochemical equipment and methods, botany equipment and methods, applications, etc., can solve the problems of antibiotic resistance gene pollution, weak adaptability of non-spore bacteria, low stability of application effect, etc. Achieve the effects of improving phytoremediation efficiency, easy transportation, and improving plant stress resistance

Active Publication Date: 2018-10-26
FUJIAN SANJU BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent 200710132243.0 provides a lead-cadmium-resistant bacterium and its phytoremediation method for soil heavy metal pollution. The soil heavy metal activation strain is Burkholderia, which is a spore-free bacterium, and a non-spore bacterium adapts to The ability is weak, and the effective live bacteria are easy to die. At the same time, the bioaccelerator made by directly filling the fermentation broth or adsorbing it with peat has a short shelf life and low stability of application effect.
Chinese patent 201310104049.7 provides an application of Trichoderma reesei combined with Sedum sedum in cadmium-contaminated farmland soil remediation. The application amount of the preparation is 4% of the test soil quality, and the application amount converted into mu is 6000kg (one mu of cultivated land) Layer soil quality is about 150,000kg), the amount is large, and the cost is high
Chinese patent 201310196985.5 provides an endophytic bacterium and its application for plant-microbe joint restoration of heavy metal-contaminated soil. The endophytic bacterium has resistance to ampicillin and tetracycline, and it is easy to apply drug-resistant strains. Potential risk of contamination with antibiotic resistance genes

Method used

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  • A soil heavy metal activating bacterial agent and its preparation method and application
  • A soil heavy metal activating bacterial agent and its preparation method and application
  • A soil heavy metal activating bacterial agent and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, bacterial strain activates the insoluble heavy metal cadmium in the fermented liquid

[0026] In order to investigate the metabolic activity of the strain on insoluble CdCO 3 Activation of 100mL culture medium in a 500mL shake flask, Trichoderma viride medium is PDB, urease bacillus medium is nutrient agar medium, jelly-like bacillus medium is ACCC55 without adding calcium carbonate Azotobacter liquid culture medium. PDB medium composition: potato 200g / L, glucose 20g / L, agar 20g / L, natural pH; nutrient agar medium composition: peptone 10g / L, beef extract 3g / L, sodium chloride 5g / L, agar 20g / L, natural pH. The ACCC55 nitrogen-fixing bacteria culture medium without adding calcium carbonate consists of: 1% sucrose, K 2 HPO 4 ·3H2 O 0.05%, NaCl 0.02%, MgSO 4 ·7H 2 O 0.02%, agar 1.8%, natural pH.

[0027] Add CdCO to shake flasks with different treatments 3 0.017g and 0.034g, so that the total cadmium concentration in the medium is 100mg / L and 200mg / L....

Embodiment 2

[0031] The preparation of embodiment 2 soil heavy metal activating bacterial agent

[0032] 1. Preparation of Trichoderma viride powder a: Activate Trichoderma viride stored in a refrigerator at 4°C to prepare a spore suspension, inoculate 1mL of Trichoderma viride spore suspension into 200mL of PDB medium, and inoculate at 28°C, Shake flask culture at 180rpm for 3 days. The composition of PDB medium is: potato 200g / L, glucose 20g / L, agar 20g / L, natural pH. The prepared Trichoderma viride seed solution was inoculated into solid-state fermentation medium at a ratio of 100 mL / kg, fermented at 30°C for 10 days, and turned once every 5 days after inoculation. The solid-state fermentation medium formula (W%) is 90% of bran, 5% of soybean meal powder, 4.7% of yeast powder, 0.1% of potassium dihydrogen phosphate, 0.1% of magnesium sulfate heptahydrate, and 0.1% of ammonium sulfate. % fresh tofu waste water and then mix and sterilize at 121°C for 1h. Collect the thalline and its fe...

Embodiment 3

[0040] Embodiment 3, heavy metal contaminated soil bioremediation test

[0041] 1 test soil

[0042] Collected from a farmland soil in a sewage irrigation area, the basic physical and chemical properties of the soil are: organic matter 19.68g / kg, available phosphorus 15.65mg / kg, available potassium 106.81mg / kg, total cadmium 4.95mg / kg, pH 7.1. After fresh soil samples pass through a 2mm sieve, add base fertilizer and apply (NH 4 ) 2 SO 4 0.71g / kg, NaH 2 PO 4 0.17g / kg and KCl 0.24g / kg, mixed well and used for pot experiments.

[0043] 2 test method

[0044] The test plant was Sedum sedum, which was collected from a lead-zinc mine in Quzhou, Zhejiang Province. The Sedum sedum was washed with ultra-pure water, cut into branches of the same size, and cultured in nutrient solution for 3 weeks in advance. After growing vigorous adventitious roots, the bioremediation test of soil heavy metals began. The culture solution is 1 / 4 Hoagland's nutrient solution.

[0045] The tes...

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Abstract

The invention discloses a soil heavy metal activating microbial inoculum and a preparation method and application thereof. Trichoderma viride, urease bacillus and bacillus mucilaginosus are high in tolerance to various heavy metals, and owing to a synergistic effect of bacterial strains, activation of the heavy metals in soil, promotion of plant absorption of the heavy metals and improvement of heavy metal pollution remediation efficiency of plants are realized. By adoption of organic wastewater as a raw material, heavy metal migration activity is further improved. Moreover, the soil heavy metal activating microbial inoculum is high in stability of application effects, the preparation method is simple and low in cost, and integration of heavy metal remediation, pest control and growth promotion for yield increase are realized.

Description

technical field [0001] The invention belongs to the technical field of heavy metal bioremediation application, and in particular relates to the preparation and application of a heavy metal activating bacterial agent. Background technique [0002] The status quo of soil heavy metal pollution in my country is not optimistic. According to the estimate of the Institute of Geographic Science and Natural Resources Research of the Chinese Academy of Sciences, the area of ​​heavy metal pollution in my country's arable land is about 180 million mu. At the press conference of the State Council Information Office at the end of December 2013, the Ministry of Land and Resources introduced that the area of ​​moderately and heavily polluted cultivated land in my country is about 50 million mu. On April 17, 2014, the Ministry of Environmental Protection and the Ministry of Land and Resources issued a national survey report on land pollution. Among the eight heavy metal pollutants of cadmium...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14B09C1/10A01N63/04A01P21/00C12R1/645C12R1/07C12R1/01
Inventor 余劲聪林克明
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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