Construction method and application of dual-system tumor screening model for communication between fruit fly organs

Abstract

The invention discloses an establishment method and application of a drosophila model for researching and screening communication signal molecules among organs to regulate and control heterogeneity tumors. According to the invention, three different fruit fly gene regulation and control expression systems, namely a Gal4 / UAS system, a QF / QUAS system and an MARCM system, are ingeniously integrated, a fruit fly adult disc specific heterogeneous tumor model (overexpression of an activated proto-oncogene Rasv12 in peripheral cells cloned by mutation of a cell polar gene script) is successfully constructed, and on the basis, Cg-Gal4 specifically expressed by a fruit fly fat body is introduced; therefore, a communication mechanism between fat body tissues and heterogeneous tumor organs can be researched in the drosophila melanogaster body. Through verification of overexpression of red fluorescent protein mCherry in adipose tissue, it is confirmed that the constructed dual-system model can play a role according to expected design. The establishment of the method provides a powerful tool for the regulation and control of the communication between the fat body and the tumor organ.

Description

Technical field [0001] The present invention relates to the field of biomedical, and more particularly to a two-system model construct and application of interior screening and research instrumental communication molecules to regulate heterogeneous tumors, and use this model to screen the fruit fling fatty body participation in regulatory heterogeneous tumors. Embarm communication molecule. Background technique [0002] Drosophila Melanogaster is an ideal model animal that studies genetic screening. Its individuals are small, easy to breed, and have a variety of genetics operating tools. A large number of genetics screening can be performed. More than 75% of human pathogenic genes can be found in fruit flies, with tumor-related signaling pathways and mammalism. The early development of Drosa larva has experienced a series of cell proliferation and growth process, and the adult tray of larvae is a common model of Drosophila research. Some carcinogens such as RAS, tumor suppressor ...

AI Technical Summary

Problems solved by technology

There have been many studies on the influence of tumors on the body, but it has not been reported whether other organs of the body such as fat body affect the tumor itself through secretory signaling molecules during tumorigenesis. The lack of research in this area is largely due to the lack of suitable Tools and Screening Models

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