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Bacillus safensis LG01 and application thereof

A technology of Bacillus suffolus and bacteria agent, which is applied to Bacillus suffolus LG01 and its application fields, can solve the problems of less research on Bacillus suffolus and the late discovery of Bacillus suffolus, and achieve the effect of broad application prospects

Active Publication Date: 2022-04-15
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the late discovery of Bacillus suffolus, there is less research on Bacillus suffolus after mutagenesis

Method used

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  • Bacillus safensis LG01 and application thereof
  • Bacillus safensis LG01 and application thereof
  • Bacillus safensis LG01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Identification of LG01 of Sandfacial Bacillus LG01

[0059] In the pollution incident of cultivating the pneumonillaries, a bacterium that had significant inhibitory effects of the pneumper collaboration. The colonies were purified by the launde in the tablet with a marker and indicated the strain number on the plate accordingly. Picking with colonies into BCYE, purifying strains using flat line. If this method does not separate strains, it is necessary to pick up a colony from the enrichment plate, and after dilution through AYE liquid medium, apply it to the medium. Then refer to the "Bojie Bacteria Identification Manual" and "Fatal Classification Identification Manual", first resolve strains belonging to bacteria, then observe the growth of colonies: Growth morphology, whether a bacteria is mixed, and whether the color is uniform, colony Individual surface and edges, etc., preliminary screening results in a candidate strain LG01, and finally the total genome se...

Embodiment 2

[0069] Example 2 Antibacterial effect of LG01 of Spike Bacillus

[0070] 1. Inhibitory effect of LG01 of Bacillus, Bacillus, Bacillus, Bacillus

[0071] (1) cultivation of strains

[0072] 1) Sandfacial pylori LG01 culture: Pick the strain preserved -80 ° C to the LB flat line, placed in a 37 ° C constant temperature incubator overnight culture, to grow on the tablet, to pick the single colony into LB liquid The medium was placed at a constant temperature shaker in 37 ° C to obtain a bacterial liquid. The bacterial liquid concentration was measured by an ultraviolet spectrometer, and the LB liquid medium was diluted to 0.01ABS to be used.

[0073] 2) White fake yeast culture: Pick the strain preserved -80 ° C to YM flat line, placed in a 30 ° C constant temperature incubator overnight culture, to grow on the tablet, and pick the single colony into YM liquid culture The group was placed at a constant temperature clocked bed culture for 24 hours to obtain a bacterial liquid. The bacte...

Embodiment 3

[0102] Example 3 Proteinase characteristics of LG01 of Bacillus LG01

[0103] Identification and measurement of LG01 secretion protease hydrolysis protein ability is based on agar hole diffusion experiment: i.e., after conflicting the milk plate, a circular sterile filter sheet having a diameter of 5 mm in the center of the plate; A 2 ul concentration of 0.01AB was added dropwise on the filter paper, and a 2 u-blank medium was added dropwise to culture 1d in a 37 ° C incubator; observe the diameter of the degradation of the plate protein. The degreasing milk plate medium used is: 5 g / l beef extract, 10 g / L protein, 1 g / LNACl, 16 g / L agar, pH 7.2.

[0104] The results showed that the strain LG01 can significantly degrade the protein, form a significant degradation ring compared to the control of the blank medium. Figure 8 ), Illuminate LG01 can be prumulin. Can be used for protease extraction, meet the needs of protease in the food industry or washing industry; can also be ...

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Abstract

The invention belongs to the technical field of microorganism application, and particularly relates to bacillus safensis LG01 and application thereof. The invention discloses bacillus safensis LG01, and the preservation number of the bacillus safensis LG01 is CCTCC (China Center for Type Culture Collection) No: M 20211624. The bacillus safensis has multiple functional characteristics: (1) the bacillus safensis has inhibitory activity on fungi such as candida albicans and aspergillus niger; (2) the compound has inhibitory activity on gram-positive bacteria such as staphylococcus aureus, micrococcus luteus and listeria monocytogenes, gram-negative bacteria such as legionella pneumophila, legionella Israeli and legionella martensii, and gram-negative plant pathogenic bacteria such as ralstonia solanacearum; (3) protease can be produced; (4) cellulase can be produced; and (5) a strong-adhesion biofilm can be formed. The functional characteristics enable the bacillus safensis to have diversified application forms, such as preparation of plant colonizers, plant biocontrol agents, bio-organic fertilizers, microbial feeds and the like, and the bacillus safensis has wide application prospects.

Description

Technical field [0001] The invention belongs to the field of microbial applications, and is specifically involved in the LG01 and its application thereof. Background technique [0002] Bacillus Safensis is a kind of Bacillus, Gram dye is positive, and intraocular. First, the spacecraft and assembly facility surface were separated from the surface of the spacecraft and assembly facility in the 2006 Jet Propulsion Lab. Thereafter, the Bacillus Saffirus was drained, and it was proved to have a variety of functions, and the application prospect was wide. [0003] First, Sandfacialus has strong resistance. There is a study to send 48 strains to the international space station, only the growth conditions of the Sandfacials JPL-Merta-8-2 in space is 60% higher than in the earth. Bacillus Saffis also has a strong metabolic role in heavy metal ions in the soil. Studies have been separated from the soil of manganese mine to obtain a patriarid B.Safensis S7, which is highly resistant to 2,2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/54C12N9/42A01N63/22A01P1/00A01P3/00A01P21/00A61K35/742A61P31/04A61P31/10C12R1/07
CPCC12N1/20C12N9/54C12N9/2437A01N63/22A61K35/742A61P31/04A61P31/10Y02A50/30
Inventor 葛振煌陆勇军陈家豪陈俊毅
Owner SUN YAT SEN UNIV
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