Method for obtaining 1, 3-diglyceride from high oleic acid sunflower seed oil

A technology for sunflower oil and diglyceride, applied in the field of obtaining 1,3-diglyceride, can solve the problem of long reaction time, undisclosed enzymatic hydrolysis of sunflower oil and its enzymatic hydrolysis products, and inability to determine whether all glycerol diols From other problems, to achieve the effect of simple operation steps and improving the efficiency of enzymatic hydrolysis

Pending Publication Date: 2022-05-03
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The unresolved problem of the above-mentioned method is: the scheme in the above-mentioned document only discloses the extraction and preparation method of sunflower oil, and does not disclose the further enzymatic hydrolysis of sunflower oil and its enzymatic products;
[0006] Although the scheme in this document discloses the extraction preparation method of sunflower oil, the following defects still exist: (1) the reaction time is too long; from sunflower oil;

Method used

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  • Method for obtaining 1, 3-diglyceride from high oleic acid sunflower seed oil
  • Method for obtaining 1, 3-diglyceride from high oleic acid sunflower seed oil
  • Method for obtaining 1, 3-diglyceride from high oleic acid sunflower seed oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1A1-A5

[0038] Embodiment 1A1-A5 focuses on the impact of deionized water with different oil-water ratios on the content of the final product 1,3-diglyceride. The specific scheme is as follows:

[0039] The method for obtaining 1,3-diglyceride from high oleic sunflower oil comprises the following steps:

[0040] (1) Enzymolysis: Accurately weigh 5g of high oleic sunflower oil, add 3000u / g Candida antarctica lipase A based on the weight of high oleic sunflower oil, and then add deionized water with different oil-water ratios (specifically See Table 1), put it in a constant temperature water bath shaker, and react at 70°C at a speed of 250r / min for 9h. After the reaction, centrifuge the product at 3000r / min for 10min, and take the supernatant to detect 1,3-DAG ( 1,3-diglyceride) content.

[0041] Table 1 Effect of different oil-water ratio (%) treatments on 1,3-DAG content

[0042]

[0043] (2) Molecular distillation: Pour 50mL of centrifuged enzymatic hydrolyzate supernatant into ...

Embodiment 1B1-B5

[0056] Embodiment 1B1-B5 focuses on the impact of water bath oscillation at different temperatures on the content of the final product 1,3-diglyceride, and the specific scheme is as follows:

[0057] The method for obtaining 1,3-diglyceride from high oleic sunflower oil comprises the following steps:

[0058] (1) Enzymolysis: Accurately weigh 7g of high oleic sunflower oil, based on the weight of high oleic sunflower oil, add 3000u / g Candida antarctica lipase A, and then add 30% deionized water was placed in a constant temperature water bath oscillator, and reacted at a speed of 250r / min for 9h under different temperature conditions (see Table 3 for details). After the reaction, the product was centrifuged at 3000r / min for 10min, and the supernatant was taken Liquid detection of 1,3-DAG (1,3-diglyceride) content.

[0059] Table 3 The effect of water bath shaking treatment at different temperatures (°C) on the content of 1,3-DAG

[0060]

[0061] (2) Molecular distillation...

Embodiment 1C1-C5

[0065] Embodiment 1C1-C5 focuses on the impact of different enzyme additions on the content of the final product 1,3-diglyceride, and the specific scheme is as follows:

[0066] The method for obtaining 1,3-diglyceride from high oleic sunflower oil comprises the following steps:

[0067] (1) Enzymolysis experiment: Accurately weigh 11 g of high oleic sunflower oil, based on the weight of high oleic sunflower oil, add different contents of Candida antarctica lipase A (see Table 4 for details), and then add High oleic sunflower oil weighs 30% deionized water, put it in a constant temperature water bath shaker, and react at a speed of 250r / min for 9h at 70°C. After the reaction, centrifuge the product at 3000r / min for 10min, and take the supernatant Liquid detection of 1,3-DAG (1,3-diglyceride) content.

[0068] Table 4 Effects of different enzyme dosage (u / g) treatments on 1,3-DAG content

[0069]

[0070] (2) Molecular distillation: Pour 50mL of centrifuged enzymatic hydro...

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Abstract

The invention belongs to the technical field of sunflower seed oil processing, and particularly relates to a method for obtaining 1, 3-diglyceride from high oleic acid sunflower seed oil. The method for obtaining the 1, 3-diglyceride from the high-oleic-acid sunflower seed oil comprises the following steps: weighing the sunflower seed oil, adding candida antarctica lipase A and deionized water, carrying out enzymolysis, constant-temperature oscillation, centrifugation and secondary molecular distillation to finally obtain a product containing the 1, 3-diglyceride, and according to the weight of the high-oleic-acid sunflower seed oil, adding the 1, 3-diglyceride into the sunflower seed oil to obtain the 1, 3-diglyceride. The dosage of the candida antarctica lipase A is 2500-3500 u/g. According to the method, sunflower seed oil is used as a main raw material, the process operation is simple, and the product is rich in nutrition and high in economic value and has wide application prospects in the industries of food, medicine, chemical engineering and the like.

Description

technical field [0001] The invention belongs to the technical field of sunflower oil processing, and in particular relates to a method for obtaining 1,3-diglyceride from high oleic sunflower oil. Background technique [0002] Regarding the method of enzymatically hydrolyzing vegetable oil, the following patent documents have been disclosed: Leng Yuxian and others disclosed a process for extracting sunflower oil by aqueous enzymatic method in the article "Aqueous Enzymatic Extraction of Sunflower Oil". The specific production process For: screening shelled sunflower seeds, cleaning and crushing, adding citrate hydrochloric acid buffer solution after crushing, stirring into a slurry, adding enzyme preparation to it according to a certain amount of enzyme: seeds, performing enzymolysis, centrifuging after enzymolysis, centrifuging Finally, free oil, emulsion layer, hydrolyzate and precipitation are obtained; the emulsion layer is taken out and subjected to secondary centrifugat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/6454
CPCC12P7/6454
Inventor 徐同成杨馨怡贾敏杜方岭宗爱珍刘丽娜黄凤洪
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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