Cell freezing medium kit and preservation method thereof

Abstract

The invention belongs to the technical field of biotechnology, and particularly relates to a cell cryopreservation liquid kit and a preservation method thereof. The large sealing cover is mounted at the opening of the shell; a placing plate is fixedly mounted in the shell; a placing hole is formed in the placing plate; a clamping piece is fixedly mounted below the placing plate; a reagent tube is inserted into the placing hole, and the reagent tube is just in contact with the bottom surface of the shell; the outer wall of the reagent tube is in contact with the clamping piece and is extruded by the clamping piece; when the reagent tube is not subjected to external force, the reagent tube is fixed relative to the position of the placing plate under the action of the clamping piece; the cell cryopreservation box is simple in structure and convenient to use, a plurality of cell cryopreservation box bodies can be stacked when being stored, the occupied space is small, meanwhile, only the reagent tubes stored in the corresponding grid areas need to be operated in the storing and taking process, and the influence on cells cryopreserved in other areas is reduced.

Description

technical field [0001] The invention belongs to the technical field of biotechnology, and specifically relates to a cell cryopreservation liquid kit and a preservation method thereof. Background technique [0002] In the process of subculture and daily maintenance of cell culture, a lot of expense is required in terms of culture equipment, culture medium and various preparations, and once the cells leave the living body to start primary culture, its various biological characteristics will gradually change and become With the increase of the number of passages and the change of the environmental conditions in vitro, there are new changes. Therefore, timely cell cryopreservation is very necessary. Cells can be stored frozen in a -70°C refrigerator for one year; cells are stored in liquid nitrogen at a temperature of -196°C, and the storage time is theoretically unlimited. At present, the commonly used program cooling box for cell cryopreservation is a circular program coolin...

AI Technical Summary

Problems solved by technology

[0004] In order to make up for the deficiencies of the existing technology, solve the problem of wasting space in the existing cell cryopreservation boxes and inconvenient single-person operation, and at the same time reduce the impact on the activity of the cells preserved in the kit during storage and retrieval, the present invention proposes a A kind of cell cryopreservation solution kit and preservation method thereof

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