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Ratio-type photoacoustic semiconductor nano polymer probe as well as preparation method and application thereof

A nano-polymer and semiconductor technology, applied in microcapsules, drug delivery, echo/ultrasound imaging agents, etc., can solve the problems of difficult real-time assessment of plaque vulnerability, false positives, unfavorable plaque areas, etc., to achieve Good biological safety, low cost, and short preparation process

Pending Publication Date: 2022-06-07
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, real-time assessment of atherosclerotic plaque vulnerability is currently elusive
[0004] Most of the existing in vivo imaging techniques for atherosclerosis are based on the signal changes caused by the reaction between probes and analytes under physiological conditions. Therefore, they will be interfered by analytes in the physiological environment (such as blood), resulting in false positive results
This is detrimental for accurate assessment of analyte concentrations in plaque areas

Method used

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  • Ratio-type photoacoustic semiconductor nano polymer probe as well as preparation method and application thereof
  • Ratio-type photoacoustic semiconductor nano polymer probe as well as preparation method and application thereof
  • Ratio-type photoacoustic semiconductor nano polymer probe as well as preparation method and application thereof

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Embodiment 1

[0039] A preparation method of a ratio-type photoacoustic semiconductor nano-polymer probe (SPN1):

[0040]Under sonication, a solution (1 mL) in tetrahydrofuran (THF) containing MDAPORBE (100-200 μg), DPP (200-400 μg) and distearoylphosphatidylethanolamine-polyethylene glycol (5-10 mg) Quickly pour into deionized water (9 mL). After sonication for 10-15 minutes, the solution was evaporated by rotary evaporator at 40-50°C to remove excess THF. Finally, the SPN1 solution can be purified 3 to 5 times by ultrafiltration.

[0041] Since MDAPROBE is conjugated to indole by rhodamine analogs, MDAPROBE alone has almost no obvious absorbance, photoacoustic and fluorescence signals due to the separation of the conjugated system due to the hydrazide structure in the structure of rhodamine analogs. When the hydrazide structure reacts with MDA, a new pyrazole structure is generated, and the generated pyrazole structure leads to the opening of the spiro ring in the structure of the rhoda...

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Abstract

The invention provides a ratio-type photoacoustic semiconductor nano polymer probe and a preparation method and application thereof, and the method comprises the following steps: under an ultrasonic condition, adding MDAPORBE, DPP and distearoyl phosphatidyl ethanolamine-polyethylene glycol into an organic solvent to obtain a mixed solution; and rapidly injecting the obtained mixed solution into deionized water, carrying out ultrasonic treatment for 10-15 minutes, evaporating through a rotary evaporator, and carrying out ultrafiltration purification. The prepared nano polymer probe can be used for detecting and imaging malondialdehyde, ratio photoacoustic imaging of malondialdehyde can be achieved through changes of absorbance and photoacoustic signals in the process, and the nano polymer probe has the advantages of being high in sensitivity, good in specificity and large in penetration depth. In addition, the nano polymer probe prepared by the invention can be used for in-vivo imaging of malonaldehyde in atherosclerotic mice so as to evaluate the vulnerability of atherosclerotic plaques.

Description

technical field [0001] The present invention relates to the technical field of biological detection, in particular, to a ratio-type photoacoustic semiconductor nano-polymer probe based on MDA response and a preparation method and application thereof. Background technique [0002] Malondialdehyde (MDA) is considered to be an important reactive toxic aldehyde biomarker for the decomposition of lipid autoxidation and peroxidation by lipid hydroperoxide. Its interactions with DNA and proteins suggest that MDA is mutagenic and carcinogenic. MDA-induced protein denaturation and DNA damage are commonly associated with a variety of human diseases, such as leukemia, diabetes, cancer, cardiovascular disease, asthma, and liver disease. However, almost all MDA detection methods, including 2-thiobarbituric acid (TBA) assay, mass spectrometry, and Raman spectroscopy, require destructive preparation of biological samples, and very few fluorescent probes have been developed recently to det...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22
CPCA61K49/225A61K49/22
Inventor 张晓兵马原宋国胜徐心昱曹晖刘柳卉
Owner HUNAN UNIV
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