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A kind of sterile porous collagen repair gel and preparation method thereof

A collagen and gel technology, applied in the field of sterile porous collagen repair gel and its preparation, can solve the problems of short degradation time, poor overall mechanical properties, poor use effect, etc., so as to induce tissue regeneration and promote tissue regeneration. repaired effect

Active Publication Date: 2022-07-29
TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, collagen products in the world are mainly divided into two categories, one is always in a solid state, such as collagen sponge made by freeze-drying or decellularized animal tissue (mainly collagen) obtained by decellularization process, through The method of surgical operation is fixed on the defect site to play the role of inducing regeneration. Another type of product is fluid, such as collagen hydrogel, collagen implant, etc., which can be injected into the body through minimally invasive surgery or injection. The degradation time of the material is short, the overall mechanical properties are poor, and the effect is not good in the defect where the body fluid is abundant

Method used

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  • A kind of sterile porous collagen repair gel and preparation method thereof
  • A kind of sterile porous collagen repair gel and preparation method thereof
  • A kind of sterile porous collagen repair gel and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A preparation method of a sterile porous collagen repair gel of the present embodiment, the method includes the following steps:

[0036] (1) Preparation of phase A: Add collagen to acetic acid with a concentration of 0.05M at 2°C, mix well, then add glycerol, and mix to obtain phase A, which is stored at 2°C, where the mass fraction of collagen in phase A is 1%, the concentration of glycerol in phase A is 0.02M;

[0037] (2) Phase B preparation: prepare a buffer solution system, the buffer solution system is a phosphate buffer solution, and the phosphate buffer solution is sodium chloride, potassium chloride, potassium dihydrogen phosphate and disodium hydrogen phosphate according to The molar ratio is 0.5:0.05:0.005:0.3, and water is added until the concentration of total phosphate is 0.8M, and the pH value is adjusted to 7.2-7.6 with hydrochloric acid and sodium hydroxide;

[0038] (3) Mix phase A and phase B before adding it to a corrosion-resistant pressure vessel...

Embodiment 2

[0040] A preparation method of a sterile porous collagen repair gel of the present embodiment, the method includes the following steps:

[0041] (1) Preparation of phase A: add collagen to acetic acid with a concentration of 0.25M at 5°C, mix well, then add mannitol and mix to obtain phase A, which is stored at 5°C, wherein the mass of collagen in phase A The fraction is 2%, and the concentration of mannitol in phase A is 0.16M;

[0042] (2) Phase B preparation: prepare a buffer solution system, the buffer solution system is a phosphate buffer solution, and the phosphate buffer solution is sodium chloride, potassium chloride, potassium dihydrogen phosphate and disodium hydrogen phosphate according to The molar ratio is 1.75:0.0275:0.0275:0.16, and water is added until the concentration of total phosphate is 1.3M, and the pH value is adjusted to 7.2-7.6 with hydrochloric acid and sodium hydroxide;

[0043] (3) After mixing phase A and phase B, add it into a corrosion-resistant...

Embodiment 3

[0045] A preparation method of a sterile porous collagen repair gel of the present embodiment, the method includes the following steps:

[0046] (1) Preparation of phase A: add collagen to acetic acid with a concentration of 0.5M at 8°C, mix well, then add catechin, and mix to obtain phase A, which is stored at 8°C. The mass fraction is 2.5%, and the concentration of catechin in phase A is 30mM;

[0047] (2) Phase B preparation: prepare a buffer solution system, the buffer solution system is a phosphate buffer solution, and the phosphate buffer solution is sodium chloride, potassium chloride, potassium dihydrogen phosphate and disodium hydrogen phosphate according to The molar ratio is 3:0.005:0.05:0.02, and water is added until the concentration of total phosphate is 1.8M, and the pH value is adjusted to 7.2-7.6 with hydrochloric acid and sodium hydroxide;

[0048] (3) Mix phase A and phase B in turn and add them to a corrosion-resistant pressure vessel, then add high-pressu...

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Abstract

The invention relates to a sterile porous collagen repair gel and a preparation method thereof, comprising the following steps: preparation of phase A: adding collagen to acetic acid or hydrochloric acid at 2-8° C., mixing evenly, adding antioxidants, and mixing to obtain A Phase B, stored at 2-8°C; Phase B preparation: prepare a buffer solution system; sequentially add Phase A and Phase B into the pressure vessel, then add high-pressure gas to the pressure vessel, seal the pressure vessel, sterilize by irradiation, 2 ‑8℃, open the pressure container when using, and obtain the sterile porous collagen repair gel. The method of the present invention adds a closed-cell structure to the collagen gel by changing the solubility of the gas in water by pressure, so as to better induce tissue regeneration. The collagen of the present invention has a specific buffer, temperature and pH value Under the environment, the collagen solution can be transformed from liquid state to gel state, and has a good clinical application prospect.

Description

technical field [0001] The invention belongs to the technical field of gels, and in particular relates to a sterile porous collagen repair gel and a preparation method thereof. Background technique [0002] Collagen, the main component of extracellular matrix, has been regarded as an ideal tissue engineering scaffold for many years due to its low immunogenicity, degradability, and ability to promote tissue regeneration. At present, collagen products in the world are mainly divided into two categories. One is always solid, such as collagen sponge made by freeze-drying or decellularized animal tissue (mainly collagen) obtained by decellularization process. The surgical method is fixed on the defect site and plays a role in inducing regeneration. Another type of product has fluidity, such as collagen hydrogels, collagen implants, etc., which can be injected into the body by minimally invasive surgery or injection. The material has a short degradation time and poor overall mech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/12C08J3/28C08L89/00A61L27/24A61L27/50A61L27/52
CPCC08J9/122C08J3/28A61L27/24A61L27/52A61L27/50A61L2400/06C08J2203/06C08J2389/00C08J2205/052C08L89/00
Inventor 孙冰冰张伊骁张娈张伟马永杰杨城斌王殷庆
Owner TIANXINFU (BEIJING) MEDICAL APPLIANCE CO LTD
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