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Yeast having high content of Abu, gamma-Glu-Abu and/or gamma-Glu-Abu-Gly

A high-content, yeast technology, applied in the direction of fermentation, enzyme, recombinant DNA technology, etc., can solve any problems such as unknown relationship

Pending Publication Date: 2022-06-24
AJINOMOTO CO INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, any relationship between acetolactate synthase activity and cellular accumulation of Abu, γ-Glu-Abu and / or γ-Glu-Abu-Gly is unknown

Method used

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  • Yeast having high content of Abu, gamma-Glu-Abu and/or gamma-Glu-Abu-Gly
  • Yeast having high content of Abu, gamma-Glu-Abu and/or gamma-Glu-Abu-Gly
  • Yeast having high content of Abu, gamma-Glu-Abu and/or gamma-Glu-Abu-Gly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0185] Of the M007Y strain and the M013Y strain ILV2 Gene mRNA expression and quantitative analysis of Abu-related compounds

[0186] (1) Culture and sampling

[0187] The M007Y strain and the M013Y strain constructed in Example 1 were inoculated into SD medium (50 mL) contained in a 500 mL volume Sakakou flask in an amount of one inoculation loop, and cultured at 30° C. for 24 hours with 120 rpm shaking.

[0188] The absorbance of each obtained culture solution was measured, and the culture solution was inoculated into SD medium (70 mL) contained in a 500 mL volume Saka-neck flask at an OD600 of 0.01, and cultured at 30°C for about 18 hours with 120 rpm Shake (main culture). Absorbance was measured by using a DU640 spectrophotometer (BECKMAN COULTER). When the OD600 became 1.5, the culture broth was sampled.

[0189] (2) M007Y strain and M013Y strain ILV2 mRNA expression analysis

[0190] The cells contained in the culture medium (4 mL) obtained by the aforementioned...

Embodiment 5

[0220] Evaluation of strains obtained by culturing in SDTE medium

[0221] Subsequently, SDTE medium was designed for evaluation of M007Y strain, M013Y strain and M014Y strain. SDTE medium was prepared as follows.

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Abstract

Provided are yeasts and yeast extracts having a high content of Abu, [gamma]-Glu-Abu, and / or [gamma]-Glu-Abu-Gly. The yeast with high content of Abu, gamma-Glu-Abu and / or gamma-Glu-Abu-Gly is obtained by modifying the yeast to reduce the activity of intracellular acetolactate synthase. A yeast extract is prepared by using the yeast obtained in this manner as a raw material.

Description

[0001] This application is the original filing date of July 9, 2014, the application number is 201480039740.4 (the international application number is PCT / JP2014 / 068283), and the name of the invention is "with high content of Abu, γ-Glu-Abu and / or γ- Glu-Abu-Gly Yeast", a divisional application of the patent application. technical field [0002] The present invention relates to yeast and yeast extracts with high content of Abu, γ-Glu-Abu and / or γ-Glu-Abu-Gly. The yeast and yeast extract are useful in the food field such as seasonings and health foods. Background technique [0003] Acetolactate synthase is known to catalyze the production of acetolactate and CO from two molecules of pyruvate 2 reaction and the formation of α-acetylhydroxybutyric acid and CO from pyruvic acid and α-butyric acid (α-KB) 2 reaction enzyme. A known ILV2 and ILV6 is the gene encoding the yeast acetolactate synthase, and ILV2 and ILV6 Encodes the catalytic subunit and the regulatory subunit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12N9/10C12N9/88C12N9/00C12P21/02A23L31/15C12R1/865C12R1/72
CPCC12N1/16C12N9/88C12N15/81A23L27/21A23L27/23A23L33/14C12Y202/01006C12N9/1022C12N9/1025A23V2002/00C12N15/815C12P13/04C12P13/14A23L33/145
Inventor 森田京西内博章
Owner AJINOMOTO CO INC