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Bifidobacterium bifidum for relieving constipation and application thereof

A Bifidobacterium bifidum and constipation technology, applied in the field of Bifidobacterium bifidum, can solve problems such as poor tolerance, and achieve the effects of accelerating small intestinal peristalsis, strong acid resistance of the strain, and repairing colonic mucosal damage

Active Publication Date: 2022-07-05
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most lactic acid bacteria have poor tolerance to the external environment. As effective probiotic strains, they must be able to pass through gastric juice with a low pH value and have a certain tolerance to bile salts.

Method used

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  • Bifidobacterium bifidum for relieving constipation and application thereof
  • Bifidobacterium bifidum for relieving constipation and application thereof
  • Bifidobacterium bifidum for relieving constipation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Isolation, Purification and Preliminary Identification of Bifidobacterium bifidum BBi32

[0107] (1) Isolation and purification of Bifidobacterium bifidum BBi32

[0108] Aspirate 1000 μL of the sample by aseptic operation and add it to 9 mL of sterile physiological saline to make a 1:10 uniform dilution, and continue to make a certain ratio of dilution. Select appropriate gradient dilutions, pipette 100 μL of each with sterile pipette tips and spread them into MRS solid plate medium respectively, incubate at 30°C for 48 hours, observe and record the colony morphology, such as figure 1 shown;

[0109] Use an inoculation loop (sterilized toothpick) to pick different colonies from the surface and interior of the plate and inoculate them into MRS liquid medium, and incubate at 30°C for 24 hours; repeat the above steps for continuous activation for 2 generations, and then carry out Gram stain microscopy on the smear, such as figure 2 As shown, it was determined that the G...

Embodiment 2

[0124] In vitro screening of Bifidobacterium bifidum BBi32

[0125] (1) Screening of probiotics tolerant to pH 3.0 artificial gastric juice.

[0126] The preparation of artificial gastric juice: 0.2% NaCl, 0.35% pepsin, adjust the pH value to 3.0 with 1 mol / L HCl, and prepare it in a sterile operating table for later use.

[0127] Determination of the tolerance of probiotics to artificial gastric juice: take 5mL of the activated strain culture solution, pour it into a sterilized 10mL centrifuge tube in a sterile operation table, collect the bacteria by centrifugation at 3000r / min for 10min, add 5mL of sterile Mix 1 mL of bacterial suspension with 9 mL of artificial gastric juice with pH 3.0, mix well, and place it in a constant temperature shaker for cultivation (37 °C, 150 r / min), and take samples at 0 h and 3 h respectively. , and poured with MRS agar medium for 48h at 37°C. The number of viable bacteria was determined by plate counting method, and the survival rate (%) wa...

Embodiment 3

[0146] Constipation model mouse experiment

[0147] 40 healthy 6-week-old female Kunming mice, weighing 26±2 g, were fed. The mice were fed with basal feed, kept in stainless steel cages, kept at room temperature of 24±2°C, relative humidity of 50±10%, and rotated between light and dark for 12 hours (lighting from 8:00 to 20:00). After 5 days of adaptation, the mice were randomly divided into 4 groups according to their body weight. group, respectively: normal group (Normal), constipation control group (Control), Bifidobacterium bifidum BBi32 low-dose group (1.0×10 7 CFU / mL), Bifidobacterium bifidum BBi32 high-dose group (1.0×10 9 CFU / mL), 10 in each group. Except for the normal group, the other groups were given loperamide 10 mg / kg by gavage, twice a day for 7 consecutive days, to establish a mouse model of constipation. The normal group was given an equal volume of normal saline for 7 consecutive days.

[0148] After the model was successfully established, the experiment ...

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Abstract

The invention provides bifidobacterium bifidum for relieving constipation and application of the bifidobacterium bifidum. The bifidobacterium bifidum for relieving constipation is named as a bifidobacterium bifidum BBi32 strain, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.16923, the preservation date is December 10, 2018, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bifidobacterium bifidum BBi32 provided by the invention can further increase the total content of short-chain fatty acids, so that the pH value of the intestinal environment is reduced, and the bifidobacterium bifidum BBi32 has the obvious characteristic of repairing colonic mucosal lesion, so that the purpose of relieving constipation is achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and in particular relates to a bifidobacteria bifidum for relieving constipation and its application. Background technique [0002] Constipation is a clinical manifestation of digestive system diseases. It is a group of symptoms with the main complaints of defecation frequency, difficulty in defecation or incomplete defecation, dry and hard stools. The prevalence of constipation is between 25 and 30%, especially in children. and common in the elderly population. After constipation occurs, stool remains in the large intestine for an extended period of time, and the water in the stool is then absorbed, resulting in dry stool, which inhibits bowel movements and causes intestinal imbalance and intestinal pain. Habitual constipation is the main culprit in inducing hyperlipidemia, cardiovascular and cerebrovascular diseases, arteriosclerosis, tumors and other diseases and th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123C12R1/01
CPCC12N1/20A23C9/1234A23C9/123A23V2400/123A23V2400/517A23V2400/249Y02A50/30
Inventor 方曙光卢容燕郭晓娟汪欣朱建国
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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