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Oral care composition

A kind of oral care and composition technology, applied in the field of oral care compositions

Pending Publication Date: 2022-07-12
COLGATE PALMOLIVE CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] While many advances have been made in the art of formulating oral care compositions to improve the ability to treat disease, more challenges remain

Method used

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Experimental program
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Embodiment 1

[0117] OKF6 cells (gingival epithelial cells) were grown in 6-well tissue culture plates. Cell monolayers were treated with the indicated doses ( figure 1 ) alone or in combination with vitamin D3 or phenylbutyrate, and cells were further incubated overnight in a tissue culture incubator. After overnight incubation, cells in RNA lysis buffer were harvested. Lysates were processed for RNA isolation using the Qiagen RNA isolation kit. RNA was processed for c-DNA preparation and further amplified (qPCR) using LL-37 (Hs 01011708_m1), Cyp24A1 (Hs00989018_m1), GAPDH (Hs 99999905_m1) Taqman gene specific probes. The QPCR data were analyzed for fold induction of LL-37 expression and the relative differences in expression were plotted. These results are described in figure 1 and 2 middle. like figure 1 and 2 As shown by the data described in, the inventive combination of the present invention provides a synergistic increase in LL-37 and CYP24A1, respectively.

Embodiment 2

[0119] Gingival tissue (GIN100) was purchased from Mattek Corporation. Tissues were treated in triplicate with vitamin D3, sodium butyrate, or phenylbutyrate alone, or a combination of the two, at the indicated doses, and tissues were incubated with these treatments in a tissue culture incubator overnight. Tissues were collected and transferred to RNA post-buffer and frozen in a -70°C freezer. When ready for processing, tissues were homogenized and processed for RNA isolation and c-DNA preparation, and qPCR amplification was performed as described above using LL-37 and GAPDH-specific Taqman probes. The results are described in Figure 3A and 3B middle. like Figure 3A and 3B As shown by the data described in, the inventive combination of the present invention provides a synergistic increase in LL-37.

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Abstract

Described herein are oral care compositions comprising vitamin D or a derivative thereof; an alkanoic acid of formula R-COOH, or an alkali metal salt thereof, wherein R is a C1 to C13 hydrocarbyl group; and an orally acceptable carrier. Methods of making and using the oral care compositions are also described.

Description

Background technique [0001] Periodontal disease is caused by the initial colonization of key pathogens such as Porphyromonas gingivalis, which lead to bacterial dysbiosis and an inflammatory response. This inflammation can eventually lead to bone loss and tooth loss, which are hallmarks of periodontal disease. Epidemiological studies have shown an association between vitamin D deficiency and chronic and invasive periodontitis. This could be due to the recently discovered relationship between vitamin D and the expression of innate immune mediators and proinflammatory cytokines. [0002] The development and validation of a cell-based screening assay to identify inducers of LL-37 is described in F. Nylén et al., Innate Immunity 2014, Vol. 20, No. 4, pp. 364-376. Innate immunity is our front line against pathogens and relies heavily on the production of antimicrobial peptides (AMPs). These peptides exhibit antimicrobial activity and immunomodulatory properties. In humans, AMPs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/36A61K8/67A61Q11/00
CPCA61Q11/00A61K8/36A61K8/67A61K8/34
Inventor 帕亚尔·阿罗拉哈什·马亨德拉·特里维迪詹姆斯·马斯特斯拉巴卜·艾哈迈德萨里塔·薇拉·梅洛塞尔吉奥·莱特
Owner COLGATE PALMOLIVE CO