Unlock instant, AI-driven research and patent intelligence for your innovation.

Plasmid used directly in intramuscular injection for treating diabetes

A technique of intramuscular injection and diabetes, applied in gene therapy, using vectors to introduce foreign genetic material, metabolic diseases, etc., can solve problems such as safety and problems

Inactive Publication Date: 2004-08-04
PEKING UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although viral vectors have the advantages of high transfection efficiency, there are safety issues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plasmid used directly in intramuscular injection for treating diabetes
  • Plasmid used directly in intramuscular injection for treating diabetes
  • Plasmid used directly in intramuscular injection for treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] 1 Construction of recombinant eukaryotic expression plasmid containing human CGRP gene

[0031] The eukaryotic expression plasmid pcDNA3.1(-)( figure 1 ) is commercially available from Invitrogen Corporation (Invitrogen Corporation, 1600 Faraday Ave. PO Box 6482, Carlsbad, CA92008, USA). The human CGRP gene is digested from the PTT42 plasmid, and the 844bp cDNA fragment containing the CGRP gene is recovered. It was connected to the downstream of the CMV promoter in the plasmid pcDNA3.1(-), and was controlled by the CMV promoter (see SEQ ID NO: 1 for the CGRP gene sequence). For the construction process of the recombinant plasmid, see figure 2 .

[0032] 2 Preparation of diabetic mouse model

[0033] Adopt classic method, select body weight 18-20 gram, the male C57BL mouse of 6-8 weeks, inject the streptozotocin (STZ) of 40mg / kg dose every day, inject continuously for 5 days, observe blood glucose and insulin concentration change ( Figure 3A and B). At 7 days, 2 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A plasmid for treating diabetes by direct intramuscular injection is disclosed. The said plasmid is the carrier pcDNA3.1(-) containing CGRP gene (CGRP-human calcitonin gene related peptide). The CGRP gene can be expressed under the pilot of CMV promoter in pc DNA 3.1 (-).

Description

technical field [0001] The invention relates to a plasmid used for direct intramuscular injection to treat diabetes. The invention directly introduces the recombinant eukaryotic expression plasmid containing the human calcitonin gene-related peptide (CGRP) gene into the skeletal muscle tissue to express the CGRP protein, realize immune regulation, and perform gene therapy on type 1 diabetes. technical background [0002] Gene therapy was born with the combination of modern medicine and molecular biology in the past ten years. It refers to introducing the target gene into the patient's body through gene transfer, so that it can make up for the mutation or expression defect of the original gene and achieve the goal of treatment. the goal of. The target gene refers to the exogenous normal gene that makes up for the functional defect. Gene transfer methods can be divided into two categories: one is the direct injection of the target gene, that is, the virus with the target gen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P3/10C12N15/85
Inventor 王宪孙威王留义
Owner PEKING UNIV