Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Organism tissue staining method

A dyeing method and biological technology, applied in biochemical equipment and methods, biological testing, microbial measurement/inspection, etc., can solve the problems of unsuitable living tissue dyeing, toxicity of dyeing reagents, cumbersome steps, etc., and achieve bright colors and outlines. Clear, time-saving results

Inactive Publication Date: 2005-03-16
PEKING UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the disadvantages of traditional staining methods are that the steps are cumbersome and time-consuming, and the samples often need special treatment; in addition, the staining reagents are highly toxic and generally not suitable for living tissue staining

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Organism tissue staining method
  • Organism tissue staining method
  • Organism tissue staining method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The fluorescent complex europium-thenoyl trifluoroacetone-triphenyl phosphine oxide was prepared into a 5 mg / L ethanol solution, and dropped onto the phospholipid. After one minute, rinse with distilled water and observe with a fluorescence microscope , The phospholipid is colored into purple clumps, as shown in Figure 1.

Embodiment 2

[0046] Using a method similar to Example 1, the mucin was stained with a terbium-ciprofloxacin solution at a concentration of 1 mg / L for 3 minutes, and bright green fluorescence appeared under ultraviolet light excitation, as shown in FIG. 2.

Embodiment 3

[0048] Using a method similar to Example 1, dye sodium nucleotide with a terbium-ofloxacin solution at a concentration of 10 mg / L for 3 minutes. Under ultraviolet light excitation, a bright red fluorescent pattern appears, as shown in Figure 3. Shown.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The present invention relates to organism tissue staining method with RE fluorescent complex. The staining reagent used in the present invention is solution of RE fluorescent complex, and the solution is dropped onto the organism tissue or the test sample is soaked in the solution for 0.5-3 min before flushing with distilled water. Under the exciting of ultraviolet light, the configuration and detailed structure of the stainless tissue sample may be observed in fluorescent microscope. The said method has simple operatino and short period, and the sample needs no pre-treatment and unlimited. The reagent has no or only weak toxicity and the staining process has no influence on the structure of tissue or cell. The tissue may be extracorporeal or intracorporeal.

Description

Technical field: [0001] The invention relates to a method for staining tissues of organisms, in particular to a method for staining tissues of dust objects by using rare earth fluorescent complexes as staining agents. Background technique: [0002] After people discovered the cells that make up the human body, understanding the microstructure of organisms represented by the human body has always been an important research field. The use of chemical substances to stain cells or tissues is an important research method, from histochemical staining represented by Hematpxylin and Epsin staining (HE) to immunohistochemistry labeled with monoclonal antibodies Staining greatly improves the resolution ability and broadens the level of observation. With the development of fluorescent reagents and fluorescence observers, fluorescence histochemistry and immunofluorescence staining are widely used in life science research with their unique colors. Field and become a hot research topic. Howeve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/00G01N1/30G01N33/48
Inventor 吴瑾光徐怡庄翁诗甫吴奇光王晶杨军杨丽敏周风山杨展澜李维红苏允兰王凡张莉任宇刘智
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com