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Rapid test and for disgnosing environmental pollution caused by pesticide and method for fabricating the card

A rapid test card and pesticide technology, applied in biochemical equipment and methods, measurement/inspection of microorganisms, analysis through chemical reactions of materials, etc., can solve the problem of poor water solubility, light color and difficult judgment of chromogenic matrix sheets and other problems, to meet the requirements of on-site diagnosis, the reaction color difference is obvious, and the result is easy to judge.

Inactive Publication Date: 2006-04-19
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of the quick test card method is that it is simple and fast, requires no training in operation, high work efficiency, and saves manpower and material resources. However, the disadvantage of the current domestic quick test card is that the color after the reaction is light, and sometimes it is not easy to judge
[0003] Found through document retrieval, "Journal of Environmental Science" volume 10 No. 1 "development and application of pesticide residue quick test box" in 1990 reported the pesticide residue quick test box developed by Li Zhixiang et al. In addition to enzyme chips and chromogenic matrix chips, there are also beakers, plastic tubes, glass rods, etc., which can be used to quickly detect organophosphorus or carbamate pesticides in vegetables, fruits, grains, and water. Chromogenic tablets are poorly water-soluble, and need to be squeezed by hand to promote dissolution
Chromogenic matrix sheets used in foreign reports also often have problems such as stability

Method used

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  • Rapid test and for disgnosing environmental pollution caused by pesticide and method for fabricating the card
  • Rapid test and for disgnosing environmental pollution caused by pesticide and method for fabricating the card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Cut the filter paper into two small discs with a diameter of 1Cm and 1.5Cm, which are used to make chromophoric film and matrix film respectively, and acetylcholinesterase, glutaraldehyde, and bovine serum albumin are respectively mixed at 1U / piece below 4°C. , 0.2μl / tablet, and 1μl / tablet are mixed with 4ml of phosphate buffer, added to carrier filter paper, soaked for 15 minutes, taken out and placed in a small fume hood to dry in the shade, that is, chromogenic tablets. When making the matrix sheet, dissolve acetyl indophenol into acetone at a concentration of 400 mg / l, soak the carrier for 15 minutes, and then place it in a small fume hood to dry in the shade. Fix two pieces of the above-mentioned chromogenic sheet and matrix sheet respectively on the diagnostic area and the reaction area of ​​the diagnostic quick test card respectively, and put 15 formed diagnostic quick test cards and 1 bottle of extraction solution into the packaging box. .

Embodiment 2

[0026] Cut the filter paper into two small discs with a diameter of 1Cm and 1.5Cm, which are used to make chromophore and matrix sheets respectively. Under 4°C, acetylcholinesterase, glutaraldehyde, and bovine serum albumin are respectively 3U / piece, Mix 0.4μl / tablet, 2μl / tablet with 4ml phosphate buffer, add carrier filter paper, soak for 15 minutes, take it out and place it in a small fume hood to dry in the shade, that is, chromogenic film. When making the matrix sheet, dissolve acetyl indophenol into acetone at a concentration of 600 mg / l, soak the carrier for 15 minutes, and then place it in a small fume hood to dry in the shade. Fix two pieces of the above-mentioned chromogenic sheet and matrix sheet respectively on the diagnostic area and the reaction area of ​​the diagnostic quick test card respectively, and put 15 formed diagnostic quick test cards and 1 bottle of extraction solution into the packaging box. .

Embodiment 3

[0028] Cut the filter paper into two small discs with a diameter of 1Cm and 1.5Cm, which are used to make chromophore and matrix sheets respectively. Under 4°C, acetylcholinesterase, glutaraldehyde, and bovine serum albumin were respectively mixed at 5U / piece, Mix 0.5μl / tablet, 3μl / tablet with 4ml phosphate buffer, add carrier filter paper, soak for 15 minutes, then take out the carrier, place it in a small fume hood to dry in the shade, that is, the chromogenic film. When making the matrix sheet, dissolve acetyl indophenol into acetone at a concentration of 800 mg / l, soak the carrier for 15 minutes, and then place it in a small fume hood to dry in the shade. Fix two pieces of the above-mentioned chromogenic sheet and matrix sheet respectively on the diagnostic area and the reaction area of ​​the diagnostic quick test card respectively, and put 15 formed diagnostic quick test cards and 1 bottle of extraction solution into the packaging box. .

[0029] Implementation Effect

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Abstract

Rapid test card includes diagnosis region made from plastic and reacting region made from paper. The diagnosis region in upper part has multiple pieces of creating colors; and the reacting region in lower part has pieces of ground substance in same quantity. Piece of creating color is prepared as following: using acetylcholine esterase in serum of insect or blood serum of animal as source of enzyme; fixing enzyme onto carrier by crosslinking method of using reagent of dual function groups, carrier protein and phosphate buffer. Piece of ground substance is prepared as following: dissolving acetyl-indophenol in ethanol or acetone, and dipping carrier of physical adsorption into the said solution, drying in vent hood. Packed kit contains shaped rapid test card including pieces of creating color and pieces of ground substance and extracting solution.

Description

technical field [0001] The invention relates to a quick test card for environmental pollution and a manufacturing method thereof, in particular to a quick test card for diagnosing pollution of organic phosphorus and carbamate pesticides in water environment and pollution-free agricultural products and a manufacturing method thereof. It belongs to the field of diagnostic testing supplies. Background technique [0002] Organophosphorus and carbamate pesticides are two types of conventional pesticides that have been used in agriculture for a long time and are highly toxic to humans and animals. With the improvement of people's living standards, the residues of pesticides in the environment, especially the residues of the above two types of pesticides, have received widespread attention. Therefore, some scholars at home and abroad are actively researching and developing new technologies and methods for rapid detection of pesticide residues on site. The Pesticide Quick Test Car...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/46G01N21/78
Inventor 陆贻通沈国清周培洪静波
Owner SHANGHAI JIAOTONG UNIV