Process for preparing thymosin

Abstract

The invention discloses a preparation method of thymosin, which belongs to the technical field of biopharmaceutical preparation. The method comprises the following steps: (1) crushing fresh thymus tissue with a high-speed shear at 20° C. to 23° C. to obtain a homogenate; (2) adding dilute hydrochloric acid to adjust the pH value between 4.5 and 4.8; (3) mixing The homogenate is placed at 20°C to 23°C for 3 to 4 hours; (4) heated in a water bath at 60°C to 65°C for 30 minutes, and naturally cooled to room temperature; (5) filtered; The product is obtained after hollow fiber column purification and sterilization. The invention has the advantages of simple and convenient method, high yield, low production cost, easy large-scale production and the like.

Description

technical field [0001] The invention relates to a preparation technology of biological medicine, in particular to a preparation method of thymosin. Background technique [0002] Thymosin has an immunomodulatory effect, and is a small molecular substance with various peptides extracted from the thymus tissue of animals by biochemical methods. The traditional thymosin preparation process and improved process usually include the following steps: (1) use a meat grinder or Colloid mill Homogenize the fresh thymus, freeze and thaw repeatedly 2 to 3 times to break up the cells; then add PH2.0 to 4.0 buffer solution, and use ultrasonic treatment below 10°C. (2) Heat the homogenate to 70°C-85°C and maintain it for 10 minutes, then centrifuge to remove the protein precipitate. (3) The centrifugate is ultrafiltered with an ultrafiltration column with a molecular weight cut-off of 6,000 to 10,000. (4) Filtrating or nanofiltration concentrating the ultrafiltrate to obtain the product. ...

AI Technical Summary

Problems solved by technology

The above-mentioned process needs to go through repeated freeze-thaw, centrifugal separation, ultrafiltration, concentration and other processes, which has the disadvantages of large equipment investment, long process time, low extraction amount and high production cost.