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Dedicated Hemolysin for flow cytometer and preparing method and application thereof

A technology of flow cytometry and hemolysin, which is applied in the field of hemolysin, can solve the problems of untimely supply, inconvenience for users, and high price, and achieve the effects of protection from infection, simple and easy production, and convenient supply

Inactive Publication Date: 2006-11-29
JINAN CENTER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the hemolysin used in flow cytometer basically relies on imports, mainly the products of Becton Dickinson (BD) and Beckman Coulter in the United States, which are expensive and cannot be supplied in time, which brings great inconvenience and waste to users

Method used

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  • Dedicated Hemolysin for flow cytometer and preparing method and application thereof
  • Dedicated Hemolysin for flow cytometer and preparing method and application thereof
  • Dedicated Hemolysin for flow cytometer and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: The composition of special hemolysin for flow cytometry is as follows:

[0045] NaCl 8g

[0046] Na 2 HPO 4 .12H 2 O 2.89g

[0047] K H 2 PO 4 0.2g

[0048] KCl0.2g

[0049] Paraformaldehyde 30g

[0050] Add double distilled water to 1L

[0051] Preparation:

[0052] 1. Weighing of raw materials: Weigh all the required raw materials according to the above formula proportions.

[0053] 2. Preparation: Mix various weighed raw materials, put them in a 50°C water bath for 4 hours, and stir and mix them every 30 minutes.

[0054] 3. Filtration: filter with double-layer filter paper to remove the precipitate.

[0055] Then carry out product sub-packaging: the filtered product is sub-packaged, 50ml / bottle. Label the packaged products, and mark the instructions for use and precautions. The finished product is accepted and put into storage.

[0056] Table 1 shows the lymphocytes and mononuclear cells obtained on the FACSCalibur flow cytomet...

Embodiment 2

[0069] The preparation method is the same as in Example 1, except that the composition of hemolysin is as follows:

[0070] NaCl 7.2g

[0071] Na 2 HPO 4 12H 2 O 3.73g

[0072] K H 2 PO 4 0.43g

[0073] Paraformaldehyde 40g

[0074] Add double distilled water to 1L

Embodiment 3

[0076] The preparation method is the same as in Example 1, except that the composition of hemolysin is as follows:

[0077] NaCl 9g

[0078] Na 2 HPO 4 12H 2 O 6g

[0079] NaH 2 PO 4 2H 2 O 0.4g

[0080] Paraformaldehyde 25g

[0081] Add double distilled water to 1L

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Abstract

It is a process method of hemolysin in special cell device and its application, which belongs to medical test agent technique field. The hemolysin is composed of NaCI, Na#-[2] HPO#-[4].12H#-[2] O, KH#-[2] PO#-[4], cavaform and double stilled water. Each component is mixed and is put in the water for four to five hours and is shaken every thirty to forty minutes and to be filtered to get the agent. The hemolysin in this invention is used as surface marks of leucocyte in blood tested by the flow cell device.

Description

(1) Technical field [0001] The invention relates to a hemolysin used in detecting the surface markers of various groups of blood leukocytes (lymphocytes, monocytes and granulocytes) by a flow cytometer, a preparation method and an application thereof. (2) Background technology [0002] Flow cytometry has been used in various fields of scientific research and medical clinical testing, such as the examination of immune function, detection of tumor antigens, transfer genes, tumor suppressor genes and other disease-related genes, apoptosis, diagnosis and prognosis of AIDS, etc. With the popularization and application of flow cytometer, the development and manufacture of its supporting reagents are becoming more and more important. At present, the hemolysin used in flow cytometry basically depends on imports, mainly products from Becton Dickinson (BD) and Beckman Coulter in the United States, which are expensive and cannot be supplied in time, causing great inconvenience and wast...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/49G01N33/50G01N1/28
Inventor 肖东杰汪运山李晓英王戒黄平张慧林刘杰
Owner JINAN CENTER HOSPITAL
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