Marine fungi polysaccharide and its extraction method and use

A technology of marine fungi and polysaccharides, applied in fungi, fermentation, drug combination, etc., to achieve the effect of inhibiting growth, easy industrial production, and inhibiting tumors

Active Publication Date: 2007-01-10
SHENZHEN NEPTUNUS PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, so far, there have been no reports on the extraction and application of polysaccharides from anti-tumor marine fungi (leptosphaeria sp.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Extraction and separation of fungal polysaccharides

[0029] Weigh 50g of mycelia, degrease with ethyl acetate and methanol respectively, add 500ml of deionized water, extract at a temperature of 70°C for 8 hours, centrifuge to remove precipitation; add ammonium sulfate with 70% of the weight of extracted water, stir and let stand for 1 hour The above precipitated protein was centrifuged to remove the precipitate; the supernatant was dialyzed against water for 72 hours to remove small molecular substances; the dialysate was concentrated to 1 / 2 of the volume of the concentrated solution, and 3 times the volume of 95% ethanol was gradually added, and stood at 4°C for 5 hours , centrifuged, and precipitated; washed with absolute ethanol, acetone, and ether in sequence, and vacuum-dried to obtain the marine fungus polysaccharide of the present invention. The content of fungal polysaccharide was determined to be 925.0 μg / mg.

Embodiment 2

[0030] Embodiment 2: Extraction and separation of fungal polysaccharides

[0031] Weigh 50g of mycelia, degrease with ethyl acetate and methanol respectively, add 1000ml of deionized water, extract at 80°C for 10 hours, centrifuge to remove precipitation; add ammonium sulfate with 85% of the weight of extracted water, stir and let stand for 1 hour The above precipitated protein was centrifuged to remove the precipitate; the supernatant was dialyzed against water for 72 hours to remove small molecular substances; the dialysate was concentrated to 1 / 2 of the volume of the concentrated solution, and 3 times the volume of 95% ethanol was gradually added, and stood at 4°C for 5 hours , centrifuged, and precipitated; washed with absolute ethanol, acetone, and ether in sequence, and vacuum-dried to obtain the marine fungus polysaccharide of the present invention. The content of fungal polysaccharide was determined to be 982.3 μg / mg.

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PUM

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Abstract

A marine fungi polysaccharide is prepared from the leptosphaeria grown in leucosolenin through defatting in weak-polarity solvent, extracting in deionized water, centrifugal separation of deposit, adding ammonium sulfate, stirring, depositing protein, centrifugal separation of deposit, adding water to supernatant, dialyzing to remove micro-molecular substances, concentrating, adding alcohol, laying aside, centrifugal separation, depositing, washing sequentially by absolute alcohol, acetone and ether, and vacuum drying. It has obvious action to suppress tumor.

Description

technical field [0001] The invention relates to a polysaccharide extracted from endophytic fungi (leptosphaeria sp.) in sponge cells and an extraction method thereof, and the application of the fungal polysaccharide in antitumor drugs. Background technique [0002] In recent years, the research of marine drugs has become a hot spot. Due to its unique living environment (such as high pressure, hyperosmosis, high temperature, low temperature and other complex and changeable ecological environments), marine organisms have formed special body structures and wonderful physiological functions, and their metabolites are different from land organisms. It provides many active substances with novel chemical structures and unique physiological functions for human beings, including terpenes, sterols, alkaloids, glycosides, polysaccharides, peptides, nucleic acids, proteins, enzymes, etc., some of which can be directly used as drugs Some of them can be used as lead compounds or molecula...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C12P19/04C12N1/14A61P35/00
Inventor 王兵张丽娟杨锐黄传贵陈红李瑞文赵金华冯汉林于琳
Owner SHENZHEN NEPTUNUS PHARM CO LTD
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