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Methods for identifying inducers and inhibitors of propteolytic antibodies, compositions and their uses

An antibody and antigen technology, applied in the fields of immunology, molecular biology and medicine, can solve problems such as increased catalytic turnover

Inactive Publication Date: 2001-06-27
内布拉斯卡大学评议会 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ensuing ΔG + 催化 A decrease in leads to an increase in turnover

Method used

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  • Methods for identifying inducers and inhibitors of propteolytic antibodies, compositions and their uses
  • Methods for identifying inducers and inhibitors of propteolytic antibodies, compositions and their uses
  • Methods for identifying inducers and inhibitors of propteolytic antibodies, compositions and their uses

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0103] f v Preparation: As outlined below, methods described in previous publications [10,14,15] were used with some adaptations. see also Figure 5 . Proceed as follows F v Construction of cDNA library: Total RNA was prepared from splenocytes of immunized mice by standard methods while minimizing RNase contamination. VL cDNA libraries were generated from RNA templates using reverse transcriptase and appropriate VL or VH forward primers (which respectively contained a SfiI restriction site and an antisense sequence encoding a peptide linker) cloned into the vector (residue 1-113) and the VH cDNA library (residues 1-123). Using Taq, dNTP and as in Figure 5 The cDNA was amplified by PCR with the appropriate primers indicated in . The reverse primer was based on the sequence encoding the conserved N-terminal amino acid in the FR1 region. A restrictive degeneracy has been introduced in the primers to allow amplification of closely related V gene families (eg kappa familie...

Embodiment

[0287] Use of CRAA and catalytic antibodies in ischemia-reperfusion injury and septic shock / SIRS Larry I requires reference to this section

[0288] Ischemia-reperfusion injury occurs when the blood supply to tissue is interrupted for a prolonged period (ischemia) and then restored (reperfusion). This type of injury affects heart attack patients and stroke patients following treatment to restore blood flow to the injured tissue. Both ischemia and especially reperfusion are associated with the release into the tissue of factors that cause inflammatory responses and injury by inducing programmed cell death.

[0289]Septic shock and systemic inflammatory response syndrome (SIRS) is the term for an often fatal syndrome that includes hemodynamic alterations, inflammation, and culminating in a predictable sequence of failure of major organs beginning with the lungs first. Septic shock syndrome was originally associated only with Gram-negative bacterial infection and the action of e...

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Abstract

Covalently reactive antigen analogs are disclosed herein. The antigens of the invention may be used to stimulate production of catalytic antibodies specific for predetermined antigens associated with particular medical disorders. The antigen analogs may also be used to permanently inactivate endogenously produced catalytic antibodies produced in certain autoimmune diseases as well as in certain lymphoproliferative disorders.

Description

field of invention [0001] The present invention relates to the fields of immunology, molecular biology and medicine. More specifically, the present invention provides novel methods and compositions for stimulating the production of novel catalytic antibodies and inhibitors thereof. The invention also provides methods for identifying and isolating naturally occurring catalytic antibodies expressed from germline genes. Finally, the present invention provides methods for the synthesis of covalently reactive antigen analogs that stimulate the production of catalytic antibodies and / or irreversibly inhibit their activity. Background of the invention [0002] In order to more fully describe the state of the art to which this invention pertains, several publications are cited throughout this application by numbers in parentheses. The specifications of each of these publications are hereby incorporated by reference. [0003] The discovery that antibodies from asthmatic patients cl...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K38/00A61K39/395A61P5/14A61P11/06A61P17/00A61P19/02A61P19/04A61P29/00A61P35/00A61P37/06C07K7/04C07K16/00C07K16/10C07K16/32C12N9/00C12N9/64
CPCC12N9/0002C07K16/1063C07K2317/34C07K16/32A61K2039/505A61P11/06A61P17/00A61P19/02A61P19/04A61P29/00A61P31/00A61P35/00A61P35/02A61P37/02A61P37/06A61P5/14C07K16/00
Inventor S·保尔G·戈洛洛波夫L·史密斯
Owner 内布拉斯卡大学评议会
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