Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof

A compound, collagen technology, applied in botanical equipment and methods, medical preparations containing active ingredients, drug combinations, etc., can solve problems such as inactivation and denaturation of heavy metal ions, achieve inhibition of bacterial infection, good flexibility, enhanced The effect of mechanical strength

Inactive Publication Date: 2003-04-02
FUXIN HUANYU RUBBER GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention adopts DNA and Ag + Combined, and then compounded with collagen to obtain a collagen-DNA-Ag complex, which solves the problem of collagen inactivation and denaturation when encountering heavy metal ions, and prepares a collagen-DNA-Ag with antibacterial function and a certain strength Complex

Method used

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  • Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof
  • Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof
  • Collagen-DNA-Ag composition with bacteriostatic function and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Dissolve 1 g of DNA in 1000 ml of deionized water precooled to 4°C under stirring. Add 1% AgNO 3 2g. Stir for 10 minutes. The solution was placed in a dialysis bag and dialyzed for 24-96h.

[0041] Take 300ml of 0.7% collagen solution, and dialyze at 4°C for 24-96h. Degas at 13.3KPa for 10 minutes. Then use a diameter of 1cm, a hole diameter of 0.4mm, and the number of holes is 50 / cm 2 Spray it into the DNA solution above under a pressure of 0.15MPa. Put the obtained gel on a 100-mesh sieve to drain the water, wash it three times with 200ml deionized water pre-cooled to 4°C, put it into a 10×10×0.5cm model, freeze at -20°C, and use P 2 o 5 , KOH as a desiccant, and vacuum-dried to obtain 10 collagen sponge films with a total weight of 3.1 g and an Ag content of 0.21% (W / W). Packaging, Co. 60 irradiated.

Embodiment 2

[0043] Dissolve 5g of DNA in 100ml of deionized water pre-cooled to 4°C under stirring, adjust the pH of the solution to 8-10, add saturated AgNO 3 0.5g, stirred for 20min, dialyzed for 24-96h.

[0044] Take 2000ml of 0.5% collagen solution, dialyze at 4°C for 24-96h, and degas at 13.3KPa for 10min. Then use a diameter of 1cm, a hole diameter of 0.5mm, and a number of holes of 50 / cm 2 Spray it into the DNA solution above under a pressure of 0.1MPa. The obtained gel was placed on a 100-mesh sieve to drain off the water, washed three times with 2000ml of deionized water pre-cooled to 4°C, and packed into a 10×10×0.5cm model, and the freezing temperature was -20°C. with P 2 o 5 , KOH as a desiccant, dry to get 55 collagen sponge films, weighing 16.2g, Ag content 0.25% (W / W), packaging, Co 60 irradiated.

Embodiment 3

[0046] Dissolve 1 g of DNA in 100 ml of deionized water pre-cooled to 4 °C under stirring, add 2% AgNO 3 Solution 1g, stirred for 10min, dialyzed for 24-96h.

[0047] Take 400ml of 0.5% collagen solution, dialyze at 4°C for 24-96h, degas at 13.3KPa for 10min, and then use 1cm diameter, 0.5mm pore size, 50 holes / cm 2 Spray it into the above DNA solution under the pressure of 0.1MPa, stir, and spray the obtained colorless transparent latex with air with a pressure of 0.5MPa and a temperature of 120°C to obtain a collagen-DNA-Ag complex Powder 1.8g, Ag content 0.20% (W / W), package, Co 60 irradiated.

[0048] Antibacterial test

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Abstract

A bacteria suppressing collagen-DNA-Ag complex is prepared from collagen, DNA and AgNO3 solution through ion exchange, dialysis, degassing, shaping, freezing, drying and sterilizing. It can be used to suppress staphylococcus aureus, Rseudomonas aeruginosa and colibacillus, and treat burn and scald.

Description

technical field [0001] The invention belongs to biochemical products, in particular to a collagen-DNA-Ag complex with antibacterial effect and a preparation method thereof. Background technique [0002] At present, there have been many studies and reports on collagen film as a medical clinical biological dressing for wound repair. Collagen extracted from animal tissues can be made into films by various methods, such as salting out film formation, ammonia fumigation film formation and freeze-drying film formation, etc. Determination, Li Xiaojian et al. Biomedical Engineering Journal 1999; 16(2); 151-153). In order to enhance the strength of the membrane, technologies such as glutaraldehyde crosslinking, double-layer bonding or impregnation of artificial or natural fibers have been used (experimental research on the production of several collagen-based wound dressings, Wu Zhigu et al. Journal of Biomedical Engineering 1999; 16(2); 147-150). However, although the collagen fi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A61K33/38A61K38/39A61P17/02
Inventor 周久才刘占龙金桂菊邢磊
Owner FUXIN HUANYU RUBBER GROUP
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